Advertisement

Highlights and Trends in Cell Encapsulation

  • Gorka Orive
  • José Luis Pedraz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 670)

Abstract

The exciting developments in the field of drug delivery have already had an enormous impact on medical technology, facilitating the administration of many drugs and improving the pharmacokinetics of many others. The past few years have also seen several firsts, including the design of novel tissue engineered approaches, intriguing advances in the fields of biomaterials and cell therapy and the improvements in the fabrication of more refined and tailored micro and nanocarriers for protein and drug delivery. The sinergy of some of these promising fields have fuelled the progress of cell encapsulation technology, a relatively old concept pioneered 60 years ago. The ability to combine cells and polymer scaffolds to create “living cell medicines” that provide longterm drug delivery has opened new doors in the use of allografts. In fact, transplanted cells may be isolated from the host’s immune system by embedding them in a permeable device that controls the outward and inward diffusion of molecules and cells. As a result of this, the requirement for immunosuppresant drugs can be eliminated or at least reduced. At present, the burgeoning number of cutting edge discoveries is leading to the design of biomimetic and biodegradable microcarriers that can easily combined with stem cells. The latter will improve the protection and transport of the cells to the target injured tissue and then promote cell integration and consequently tissue repair or regeneration.

In the present reviews, we discussed the state of the art in the field of cell encapsulation technology. This book describes the most relevant aspects of the design and development of cell-loaded microcapsules. Some of the most interesting therapeutic applications of this technology are presented as are some of the limitations, future challenges and directions in the field.

Keywords

Alginate Hydrogel Cell Encapsulation Cell Microencapsulation Tissue Engineer Approach Microencapsulation Technology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    De Vos P, Bucko M, Gemeiner P et al. Multiscale requirements for bioencapsulation in medicine and biotechnology. Biomaterials 2009; 30:2559–2570.CrossRefPubMedGoogle Scholar
  2. 2.
    Dornenburg H. Evaluation of immobilisation effects on metabolic activities and productivity in plant cell processes. Proc Biochem 2004; 39:1369–1375.CrossRefGoogle Scholar
  3. 3.
    Doleyres Y, Lacroix C. Technologies with free and immobilised cells for probiotic bifidobacteria production and protection. Int Dairy J 2005; 15:973–988.CrossRefGoogle Scholar
  4. 4.
    Guerin D, Vuillemard JC, Subirade M. Protection of bifidobacteria encapsulated in polysaccharide-protein gel beads against gastric juice and bile. J Food Prot 2003; 66:2076–2084.PubMedGoogle Scholar
  5. 5.
    Urbanska AM, Bhathena J, Prakash S. Live encapsulated Lactobacillus acidophilus cells in yogurt for therapeutic oral delivery: preparation and in vitro analysis of alginate-chitosan microcapsules. Can J Physiol Pharmacol 2007; 85:884–893.CrossRefPubMedGoogle Scholar
  6. 6.
    Orive G, Hernández RM, Gascón AR et al. Cell encapsulation: promise and progress. Nat Med 2003; 9:104–107.CrossRefPubMedGoogle Scholar
  7. 7.
    Orive G, Hernández RM, Gascón AR et al. History, challenges and perspectives of cell microencapsulation. Trends Biotechnol 2004; 22:87–92.CrossRefPubMedGoogle Scholar
  8. 8.
    Koo J, Chang TSM. Secretion of erythropoietin from microencapsulated rat kidney cells. Int J Artif Organs 1993; 16:557–560.PubMedGoogle Scholar
  9. 9.
    Chang PL, Shen N, Westcott AJ. Delivery of recombinant gene products with microencapsulated cells in vivo. Hum Gene Ther 1993; 4:433–440.CrossRefPubMedGoogle Scholar
  10. 10.
    Liu HW, Ofosu FA, Chang PL. Expression of human factor IX by microencapsulated recombinant fibroblasts. Hum Gene Ther 1993; 4:291–301.CrossRefPubMedGoogle Scholar
  11. 11.
    Cieslinski DA, Humes HD. Tissue engineering of a bioartificial kidney. Biotechnol Bioeng 1994; 43:678–681.CrossRefPubMedGoogle Scholar
  12. 12.
    Wong H, Chang TM. Bioartificial liver: implanted artificial cells microencapsulated living hepatocytes increases survival of liver failure rats. Int J Artif Organs 1986; 9:335–336.PubMedGoogle Scholar
  13. 13.
    Aebischer P, Russell PC, Christenson L et al. A bioartificial parathyroid. ASAIO Trans 1986; 32:134–137.CrossRefPubMedGoogle Scholar
  14. 14.
    Aebischer P, Goddard M, Signore AP et al. Functional recovery in hemiparkinsonian primates transplanted with polymer-encapsulated PC12 cells. Exp Neurol 1994; 126:151–158.CrossRefPubMedGoogle Scholar
  15. 15.
    Lim F, Sun AM. Microencapsulated islets as bioartificial endocrine pancreas. Science 1980; 210:908–910.CrossRefPubMedGoogle Scholar
  16. 16.
    Barnett BP, Kraitchman DL, Lauzon C et al. Radiopaque alginate microcapsules for X-ray visualization and immunoprotection of cellular therapeutics. Mol Pharm 2006; 3:531–538.CrossRefPubMedGoogle Scholar
  17. 17.
    Siti-Ismail N, Bishop AE, Polak JM et al. The benefit of human embryonic stem cell encapsulation for prolonged feeder-free maintenance. Biomaterials 2008; 29:3946–3952.CrossRefPubMedGoogle Scholar
  18. 18.
    Haiying Yu, Pamela J. VandeVord, Li Mao. Improved tissue-engineered bone regeneration by endothelial cell mediated vascularisation. Biomaterials 2009; 30:508–517.CrossRefGoogle Scholar

Copyright information

© Landes Bioscience and Springer Science+Business Media 2010

Authors and Affiliations

  • Gorka Orive
    • 1
  • José Luis Pedraz
    • 1
  1. 1.Faculty of Pharmacy, Laboratory of Pharmacy and Pharmaceutical TechnologyUniversity of the Basque CountryVitoria GasteizSpain

Personalised recommendations