Techniques to Determine Clonality in Hematolymphoid Malignancies

  • Daniel E. Sabath
Part of the Molecular Pathology Library book series (MPLB, volume 4)


Clonality testing is a well-established molecular diagnostic technique in the realm of hematopathology. Molecular techniques to diagnose hematological malignancies based on the detection of clonality have been with us since the mid-1980s, based on the seminal observation that lymphoid cells rearrange their antigen receptor genes and that recurrent genetic rearrangements are present in myeloid neoplasms. In this chapter, I review the history of clonality testing in cancer, describe the scientific principles on which clonality-based testing rest, and then describe the numerous techniques that are now available for diagnosing hematolymphoid malignancies by demonstrating their clonal nature.


Cell Clonality Antigen Receptor Androgen Receptor Gene FLT3 Mutation Clonal Nature 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Daniel E. Sabath
    • 1
  1. 1.Departments of Laboratory Medicine and MedicineUniversity of Washington School of MedicineSeattleUSA

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