Molecular Techniques to Detect Disease and Response to Therapy: Minimal Residual Disease

  • Marie E. Beckner
  • Jeffrey A. Kant
Part of the Molecular Pathology Library book series (MPLB, volume 4)


Evaluation for the presence of neoplastic cells or karyotypic abnormalities has traditionally been performed to monitor therapeutic response of hematolymphoid neoplasms. The application of multicolor flow cytometry and nucleic acid amplification techniques has extended evaluable markers and lowered limits of detection, thus leading to the term “minimal residual disease” (MRD). The ability to monitor MRD has, in turn, led to new concepts in the definition of disease “remission” and early relapse and opportunities for personalized therapy. This chapter focuses on the principles of the molecular assessment of MRD.


Acute Myeloid Leukemia Minimal Residual Disease Fusion Transcript Acute Myeloid Leukemia Case Nucleic Acid Amplification Technique 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Marie E. Beckner
    • 1
  • Jeffrey A. Kant
    • 2
  1. 1.Molecular Diagnostics, Department of PathologyUniversity of PittsburghPittsburghUSA
  2. 2.Division of Molecular Diagnostics, Department of Pathology and Human GeneticsUniversity of Pittsburgh Medical CenterPittsburghUSA

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