Ontogeny of Cl- Homeostasis in Mouse Hypoglossal Nucleus
We tested the immunoreactivity of KCC2 using KCC2 antibody in the developmental mouse medulla. Age-dependent changes in immunoreactivity were remarkable in the hypoglossal nucleus, and interestingly, the immunoreactivity in the hypoglossal nucleus relative to the dorsal vagal nucleus at P0 appeared to be higher than that of P7. Thus Cl− homeostasis in the hypoglossal nucleus might be differentially regulated in the developmental stage.
KeywordsH2O2 Sucrose Paraformaldehyde Barbiturate
This research was partially supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Young Scientists (B), 21791046, 2009.
- Hsu, S.M., Raine, L., and Fanger, H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 557–580.Google Scholar