Antioxidant Treatment Does Not Prevent Chronic Hypoxia-Induced Respiratory Muscle Impairment in Developing Rats

  • Jayne Carberry
  • Aidan Bradford
  • Ken D. O’Halloran
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)


Litters of rats were exposed to normobaric normoxia or hypobaric hypoxia (PB= 450 mmHg) for 7 days at 3 different time points during early development (postnatal day (P)1, P6 & P11). A separate litter exposed to hypoxia at P11 was treated with the antioxidant Tempol (100 mg/kg) given by oral administration daily starting at P8. At P19, sternohyoid and diaphragm muscles were removed and isolated muscle bundles were mounted isometrically in physiological salt solution at 30°C in vitro. Fatigue was assessed in response to repeated stimulation (40 Hz) every 2 s for 5 min. Fatigue index was measured. Chronic hypoxia decreased sternohyoid, but not diaphragm, muscle endurance. Tempol treatment did not prevent hypoxia-induced muscle plasticity, suggesting that reactive oxygen species are not implicated in hypoxia-induced muscle dysfunction.


Respiratory Muscle Chronic Hypoxia Hypobaric Hypoxia Muscle Dysfunction Diaphragm Muscle 
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Supported by the Health Research Board, Ireland and UCD.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Jayne Carberry
    • 1
  • Aidan Bradford
    • 2
  • Ken D. O’Halloran
    • 1
  1. 1.UCD School of Medicine and Medical ScienceUniversity College DublinDublin 4Ireland
  2. 2.Department of Physiology and Medical PhysicsRoyal College of Surgeons in IrelandDublin 2Ireland

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