Abstract
In vivo, diffusional O2 gradients from the capillary blood to the intracellular space determine O2 availability at the O2 sensing molecules in the cell. With a novel technique for imaging intracellular O2 levels using green fluorescent protein (GFP), we examined the possibility that diffusional O2 concentration gradients might be involved in the cellular hypoxic sensing in cultured Hep3B cells. In the present study, we failed to demonstrate significant gradients of intracellular O2 when mitochondrial respiration was maximally elevated by an uncoupler of oxidative phosphorylation. Thus, we conclude that intracellular O2 gradients may be negligible at normal mitochondrial O2 demand in these cells.
Keywords
- Green Fluorescent Protein
- Mitochondrial Respiration
- Hep3B Cell
- Green Fluorescent Protein Fluorescence
- Cell Disk
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Aragonés, J., Schneider, M., Van Geyte, K. et al. (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabolism. Nat. Genet. 40, 170–180.
Berchner-Pfannschmidt, U., Tug, S., Trinidad, B. et al. (2008) Nuclear oxygen sensing: Induction of endogenous prolyl-hydroxylase 2 activity by hypoxia and nitric oxide. J. Biol. Chem. 283, 31745–31753.
Elowitz, M.B., Surette, M.G., Wolf, P.E. et al. (1997) Photoactivation turns green fluorescent protein red. Curr. Biol. 7, 809–812.
Kim, J.W., Tchernyshyov, I., Semenza, G.L. et al. (2006) HIF-1-mediated expression of pyruvate dehydrogenase kinase: A metabolic switch required for cellular adaptation to hypoxia. Cell Metab. 3, 177–185.
Lu, C.W., Lin, S.C., Chen, K.F. et al. (2008) Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance. J. Biol. Chem. 283, 28106–28114.
Papandreou, I., Cairns, R.A., Fontana, L. et al. (2006) HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption. Cell Metab. 3, 187–197.
Semenza, G.L. (2009) Regulation of oxygen homeostasis by hypoxia-inducible factor 1. Physiology (Bethesda) 24, 97–106.
Sridharan, V., Guichard, J., Li, C.Y. et al. (2008) O2-sensing signal cascade: Clamping of O2 respiration, reduced ATP utilization, and inducible fumarate respiration. Am. J. Physiol. Cell Physiol. 295, C29–C37.
Takahashi. E., Takano, T., Nomura, Y. et al. (2006) In vivo oxygen imaging using green fluorescent protein. Am. J. Physiol. Cell Physiol. 291, C781-C787.
Vaupel, P. (2004) Tumor microenvironmental physiology and its implications for radiation oncology. Semin. Radiat. Oncol. 14, 198–206
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Takahashi, E., Sato, M. (2010). Intracellular Diffusion of Oxygen and Hypoxic Sensing: Role of Mitochondrial Respiration. In: Homma, I., Onimaru, H., Fukuchi, Y. (eds) New Frontiers in Respiratory Control. Advances in Experimental Medicine and Biology, vol 669. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5692-7_43
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DOI: https://doi.org/10.1007/978-1-4419-5692-7_43
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