Vesicular Glutamate Transporter 2-Immunoreactive Synapses onto Phrenic Motoneurons in the Neonatal Rat

  • Shigefumi Yokota
  • Yoshio Shinozaki
  • Yoshitaka Oku
  • Yasumasa Okada
  • Yukihiko Yasui
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)


The synaptic organization between vesicular glutamate transporter 2 (VGLUT2)-immunoreactive (ir) axon terminals and phrenic motoneurons in the neonatal rat was examined using a combined retrograde tracing and immunohistochemistry for VGLUT2. The phrenic nucleus (PhN) contained large numbers of VGLUT2-ir axon terminals, some of which made axosomatic and axodendritic synapses with PhN motoneurons. These terminals were of asymmetrical type and contained spherical clear synaptic vesicles. The results suggest that in the neonatal rat glutamatergic synapses onto PhN motoneurons exist and mediate excitatory transmission to drive PhN motoneurons.


Axon Terminal Normal Donkey Serum Vesicular Glutamate Transporter Neonatal Animal Axodendritic Synapse 
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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shigefumi Yokota
    • 1
  • Yoshio Shinozaki
    • 2
  • Yoshitaka Oku
    • 3
  • Yasumasa Okada
    • 4
  • Yukihiko Yasui
    • 1
  1. 1.Department of Anatomy a Morphological NeuroscienceShimane University School of MedicineIzumoJapan
  2. 2.Department of Orthopaedic SurgeryHino Municipal HospitalTokyoJapan
  3. 3.Department of PhysiologyHyogo College of MedicineHyogoJapan
  4. 4.Department of MedicineKeio University Tsukigase Rehabilitation CenterIzu CityJapan

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