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Vesicular Glutamate Transporter 2-Immunoreactive Synapses onto Phrenic Motoneurons in the Neonatal Rat

  • Shigefumi Yokota
  • Yoshio Shinozaki
  • Yoshitaka Oku
  • Yasumasa Okada
  • Yukihiko Yasui
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)

Abstract

The synaptic organization between vesicular glutamate transporter 2 (VGLUT2)-immunoreactive (ir) axon terminals and phrenic motoneurons in the neonatal rat was examined using a combined retrograde tracing and immunohistochemistry for VGLUT2. The phrenic nucleus (PhN) contained large numbers of VGLUT2-ir axon terminals, some of which made axosomatic and axodendritic synapses with PhN motoneurons. These terminals were of asymmetrical type and contained spherical clear synaptic vesicles. The results suggest that in the neonatal rat glutamatergic synapses onto PhN motoneurons exist and mediate excitatory transmission to drive PhN motoneurons.

Keywords

Axon Terminal Normal Donkey Serum Vesicular Glutamate Transporter Neonatal Animal Axodendritic Synapse 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Ellenberger, H.H., Feldman, J.L., and Goshgarian, H.G. (1990a) Ventral respiratory group projections to phrenic motoneurons: Electron microscopic evidence for monosynaptic connections. J. Comp. Neurol. 302, 707–714.CrossRefPubMedGoogle Scholar
  2. Ellenberger, H.H., Vera, P.L., Haselton, J.R., Haselton, C.L., and Schneiderman, N. (1990b) Brainstem projections to the phrenic nucleus: an anterograde and retrograde HRP study in the rabbit. Brain. Res. Bull. 24, 163–174.CrossRefPubMedGoogle Scholar
  3. Greer, J.J., Smith, J.C., and Feldman, J.L. (1991) Role of excitatory amino acids in the generation and transmission of respiratory drive in neonatal rat. J. Physiol. 437, 727–749.PubMedGoogle Scholar
  4. Greer, J.J., Smith, J.C., and Feldman, J.L. (1992) Glutamate release and presynaptic action of AP4 during inspiratory drive to phrenic motoneurons. Brain. Res. 576,355–357.CrossRefPubMedGoogle Scholar
  5. Lindsay, A.D., Greer, J.J., and Feldman, J.L. (1991) Phrenic motoneuron morphology in the neonatal rat. J. Comp. Neurol. 308, 169–179.CrossRefPubMedGoogle Scholar
  6. Liu, G., Feldman, J.L., and Smith, J.C. (1990) Excitatory amino acid-mediated transmission of inspiratory drive to phrenic motoneurons. J. Neurophysiol. 64, 423–436.PubMedGoogle Scholar
  7. McCrimmon, D.R., Smith, J.C., and Feldman, J.L. (1989) Involvement of excitatory amino acids in neurotransmission of inspiratory drive to spinal respiratory motoneurons. J. Neurosci. 9, 1910–1921.PubMedGoogle Scholar
  8. Murphy, S.M., Pilowsky, P.M., and Llewellyn-Smith, I.J. (1996) Vesicle shape and amino acids in synaptic inputs to phrenic motoneurons: do all inputs contain either glutamate or GABA? J. Comp. Neurol. 373, 200–219.CrossRefPubMedGoogle Scholar
  9. Stornetta, R.L., Sevigny, C.P., and Guyenet, P.G. (2003) Inspiratory augmenting bulbospinal neurons express both glutamatergic and enkephalinergic phenotypes. J. Comp. Neurol. 455, 113–124.CrossRefPubMedGoogle Scholar
  10. Tai, Q., and Goshgarian, H.G. (1996) Ultrastructural quantitative analysis of glutamatergic and GABAergic synaptic terminals in the phrenic nucleus after spinal cord injury. J. Comp. Neurol. 372, 343–355.CrossRefPubMedGoogle Scholar
  11. Yokota, S., Oka, T., Tsumori, T., Nakamura, S., and Yasui, Y. (2007) Glutamatergic neurons in the Kölliker-Fuse nucleus project to the rostral ventral respiratory group and phrenic nucleus: A combined retrograde tracing and in situ hybridization study in the rat. Neurosci. Res. 59, 341–346.CrossRefPubMedGoogle Scholar
  12. Yokota, S., Tsumori, T., Ono, K., and Yasui, Y. (2004) Glutamatergic pathways from the Kölliker-Fuse nucleus to the phrenic nucleus in the rat. Brain. Res. 995, 118–130.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shigefumi Yokota
    • 1
  • Yoshio Shinozaki
    • 2
  • Yoshitaka Oku
    • 3
  • Yasumasa Okada
    • 4
  • Yukihiko Yasui
    • 1
  1. 1.Department of Anatomy a Morphological NeuroscienceShimane University School of MedicineIzumoJapan
  2. 2.Department of Orthopaedic SurgeryHino Municipal HospitalTokyoJapan
  3. 3.Department of PhysiologyHyogo College of MedicineHyogoJapan
  4. 4.Department of MedicineKeio University Tsukigase Rehabilitation CenterIzu CityJapan

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