Influence of Extracellular [K+]o on Inspiratory Network Complexity of Phrenic and Hypoglossal Nerve Discharge in Arterially-Perfused Adult Rat

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)


Many in vitro mammalian preparations are used to study multiple aspects of central respiratory control. In these preparations, recordings of respiratory-related outputs that range from individual and population neuronal activities to hypoglossal (XII) nerve output to phrenic (PHR) nerve discharge commonly are used. These reduced preparations typically are supplied with an artificial cerebral spinal fluid (aCSF) containing an extracellular potassium level ([K+]o) elevated above physiological levels in order to increase excitability and maintain a stable respiratory output. To begin to investigate the effects of [K+]o on the relationship between PHR and XII phase components, as well as the complexity underlying their respiratory-related network components, we examined the effects of various [K+]o levels on simultaneously recorded PHR and XII nerve activities in an arterially-perfused adult rat preparation.


Choline Chloride Extracellular Potassium Nerve Discharge Artificial Cerebral Spinal Fluid Medullary Slice 
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This work was supported by NIH grants NS045321 and NS049310.


  1. Pincus, S.M. (1991) Approximate entropy as a measure of system complexity. Proc. Natl. Acad. Sci. USA. 88(6), 2297–2301.CrossRefPubMedGoogle Scholar
  2. Solomon, I.C., Rodriguez, M.N., and Chon, K.H. (2003) Blockade of brain stem gap junctions increases phrenic burst frequency and reduces phrenic burst synchronization in adult rat. J. Neurophysiol. 89, 135–149.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Tabitha Y. Shen
    • 1
  • Kenichi Ono
    • 1
  • Irene C. Solomon
    • 1
  1. 1.Department of Physiology and BiophysicsStony Brook UniversityStony BrookUSA

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