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Loss of Pre-Inspiratory Neuron Synchroneity in Mice with DSCAM Deficiency

  • Kenji Amano
  • Morimitsu Fujii
  • Satoru Arata
  • Masaharu Ogawa
  • Kazuhiro Yamakawa
  • Akiko Arata
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 669)

Abstract

Down syndrome cell adhesion molecule (DSCAM) is a neural adhesion molecule that plays diverse roles in neural development. We disrupted the Dscam locus in mice and found that the null mutants (Dscam -/-) died within 24 hours after birth. Whole body plethysmography showed irregular respiration and lower ventilatory response to hypercapnia in the null mutants. Further, a medulla-spinal cord preparation of Dscam -/- mice showed that the C4 ventral root activity, which drives diaphragm contraction for inspiration, had an irregular rhythm with frequent apneas. Optical imaging of the preparation using voltage-sensitive dye revealed that the pre-inspiratory (Pre-I) neurons located in the rostral ventrolateral medulla (RVLM) and belonging to the rhythm generator for respiration, lost their synchroneity in Dscam -/- mice. Dscam +/− mice, which survived to adulthood without any overt abnormalities, also showed irregular respiration but milder than Dscam -/- mice. These results suggest that DSCAM plays a critical role in central respiratory regulation in a dosage-dependent manner. These results have been published (Amano et al. 2009).

Keywords

Down Syndrome Facial Nerve Activity Facial Nucleus Rostral Ventrolateral Medulla Ventral Medulla 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was partly supported by a grant from RIKEN Brain Science Institute, Grant-in-Aid for Scientific Research (KAKENHI) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology. We thank Dr. T. Takeuchi, N. Nishiyama, and Y. Onodera for technical support, Dr. K. Yamaguchi for helpful suggestions.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kenji Amano
    • 1
  • Morimitsu Fujii
    • 2
  • Satoru Arata
    • 3
  • Masaharu Ogawa
    • 4
  • Kazuhiro Yamakawa
    • 1
  • Akiko Arata
    • 2
    • 5
  1. 1.Laboratory for NeurogeneticsRIKEN Brain Science InstituteSaitamaJapan
  2. 2.Laboratory for Memory and LearningRIKEN Brain Science InstituteSaitamaJapan
  3. 3.Center for BiotechnologyShowa UniversityTokyoJapan
  4. 4.Laboratory for Cell Culture DevelopmentRIKEN Brain Science InstituteSaitamaJapan
  5. 5.Department of PhysiologyHyogo College of MedicineHyogoJapan

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