Testis Tissue Xenografting

  • Jose R. Rodriguez-Sosa
  • Stefan Schlatt
  • Ina Dobrinski


Testis tissue xenografting involves a series of procedures from donor tissue preparation to graft recovery for analysis and/or sperm harvesting. In the current section, we describe these protocols in detail (modified from Dobrinski and Rathi [1]).


Donor Tissue Testis Tissue Testicular Artery Midline Skin Incision Plastic Tissue Culture Dish 


  1. 1.
    Dobrinski I, Rathi R. Ectopic grafting of mammalian testis tissue into mouse hosts. In: Hou SX, Singh SR, editors. Methods in molecular biology germ line stem cells. Totowa: Humana Press; 2008. p. 139–48.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jose R. Rodriguez-Sosa
    • 2
  • Stefan Schlatt
    • 3
  • Ina Dobrinski
    • 1
  1. 1.Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary MedicineUniversity of CalgaryCalgaryCanada
  2. 2.Department of Comparative Biology and Experimental MedicineUniversity of CalgaryCalgaryCanada
  3. 3.Center of Reproductive Medicine and AndrologyUniversity Hospital of MünsterMünsterGermany

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