SuperMEN1 pp 105-115 | Cite as

Functional Studies of Menin through Genetic Manipulation of the Men1 Homolog in Mice

  • Dheepa Balasubramanian
  • Peter C. Scacheri
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 668)


To investigate the physiological role of menin, the protein product of the MEN1 gene, several groups have utilized gene targeting strategies to delete one or both copies of the mouse homolog Men1. Mice that arehomozygous null for Men1 die duringembryogenesis. Heterozygous Men1 mice are viable and develop many of the same types of tumors as humans with MEN1. In addition to conventional knockouts of Men1, tissue-specificelimination of menin using ere-lox has been achieved in pancreatic β cells, anterior pituitary, parathyroid, liver, neural crest and bone marrow, with varying results that are dependent on cell context. In this chapter, we compare the phenotypes of the different conventional Men1 knockouts, detail the similarities and differences between Men1 pathogenesis in mice and humans and highlight results from recent crossbreeding studies between Men1 mutants and mice with null mutations in genes within the retinoblastoma pathway, including p18 Inc4c , p27 Kip1 and Rb. In addition, we discuss not only how the Men1 mutants have shed light on the role of menin in endocrine tumor suppression, but also how Men1 mutant mice have helped uncover previously unrecognized roles for menin in development, leukemogenesis and gestational diabetes.


Pituitary Adenoma Neural Crest Multiple Endocrine Neoplasia Type Parathyroid Adenoma Parathyroid Tumor 
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Copyright information

© Landes Bioscience and Springer Science+Business Media 2009

Authors and Affiliations

  • Dheepa Balasubramanian
    • 1
  • Peter C. Scacheri
    • 1
  1. 1.Department of GeneticsCaseWestern Reserve UniversityClevelandUSA

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