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A Glimpse into the Proteome of Phototrophic Bacterium Rhodobacter capsulatus

  • Ozlem Onder
  • Semra Aygun-Sunar
  • Nur Selamoglu
  • Fevzi Daldal
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 675)

Abstract

A first glimpse into the proteome of Rhodobacter capsulatus revealed more than 450 (with over 210 cytoplasmic and 185 extracytoplasmic known as well as 55 unknown) proteins that are identified with high degree of confidence using nLC–MS/MS analyses. The accumulated data provide a solid platform for ongoing efforts to establish the proteome of this species and the cellular locations of its constituents. They also indicate that at least 40 of the identified proteins, which were annotated in genome databases as unknown hypothetical proteins, correspond to predicted translation products that are indeed present in cells under the growth conditions used in this work. In addition, matching the identification labels of the proteins reported between the two available R. capsulatus genome databases (ERGO-light with RRCxxxxx and NT05 with NT05RCxxxx numbers) indicated that 11 such proteins are listed only in the latter database.

Keywords

Major Metabolic Pathway Acclaim PepMap100 Tryptic Peptide Mixture Comprehensive Proteome Analysis Predict Translation Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work is supported by grants from DOE ER9120053 and NIH GM38237 to F.D.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ozlem Onder
    • 1
  • Semra Aygun-Sunar
    • 1
  • Nur Selamoglu
    • 1
  • Fevzi Daldal
    • 1
  1. 1.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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