Food Analysis pp 283-299 | Cite as

Application of Enzymes in Food Analysis

Part of the Food Analysis book series (FSTS)


Enzymes are protein catalysts that are capable of very great specificity and reactivity under physiological conditions. Enzymatic analysis is the measurement of compoundswith the aid of added enzymes or themeasurement of endogenous enzyme activity to give an indication of the state of a biological system including foods. The fact that enzyme catalysis can take place under relatively mild conditions allows for measurement of relatively unstable compounds not amenable to some other techniques. In addition, the specificity of enzyme reactions can allow for measurement of components of complex mixtures without the time and expense of complicated chromatographic separation techniques.


Substrate Concentration Glucose Oxidase Enzyme Concentration Apple Juice Burk Plot 


  1. 1.
    Powers JR, Whitaker JR (1977) Effect of several experimental parameters on combination of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor with porcine pancreatic α-amylase. J Food Biochem 1:239CrossRefGoogle Scholar
  2. 2.
    Whitaker JR (1985) Analytical uses of enzymes. In: Gruenwedel D, Whitaker JR (eds) Food Analysis. Principles and Techniques, vol 3. Biological Techniques, Marcel Dekker, New York, pp 297–377Google Scholar
  3. 3.
    Bernfeld P (1955) Amylases, α and β. Methods Enzymol 1:149CrossRefGoogle Scholar
  4. 4.
    Henniger G (2004) Nonindustrial enzyme usage: Enzymes in food analysis. In: Aehle W (ed) Enzymes in Industry, Production and Applications, 2nd edn, Wiley-VCH Verlag GmbH & Co. Weinheim, Germany, pp 322–334Google Scholar
  5. 5.
    Bergmeyer HU (1983) Methods of Enzymatic Analysis. Academic Press, New YorkGoogle Scholar
  6. 6.
    Beutler H (1984) A new enzymatic method for determination of sulphite in food. Food Chem 15:157CrossRefGoogle Scholar
  7. 7.
    Raabo E, Terkildsen TC (1960) On the enzyme determination of blood glucose. Scand J Clin Lab Invest 12:402CrossRefGoogle Scholar
  8. 8.
    Beutler H, Wurst B (1990) A new method for the enzymatic determination of d-malic acid in foodstuffs. Part I: Principles of the Enzymatic Reaction. Deutsche Lebensmittel-Rundschau 86:341Google Scholar
  9. 9.
    USDA (1975) Enzyme inactivation tests (frozen vegetables). Technical inspection procedures for the use of USDA inspectors. Agricultural Marketing Service, U.S. Department of Agriculture, Washington, DCGoogle Scholar
  10. 10.
    Williams DC, Lim MH, Chen AO, Pangborn, RM, Whitaker JR (1986) Blanching of vegetables for freezing – Which indicator enzyme to use. Food Technol 40(6): 130.Google Scholar
  11. 11.
    Surrey K (1964) Spectrophotometric method for determination of lipoxidase activity. Plant Physiology 39:65CrossRefGoogle Scholar
  12. 12.
    Zhang Q, Cavalieri, RP, Powers JR, Wu J (1991) Measurement of lipoxygenase activity in homogenized green bean tissue. J Food Sci 56:719CrossRefGoogle Scholar
  13. 13.
    Murthy GK, Kleyn DH, Richardson T, Rocco RM (1992) Phosphatase methods. In: Richardson GH (ed) Standard methods for the examination of dairy products, 16th edn. American Public Health Association, Washington, DC, p. 413Google Scholar
  14. 14.
    Rocco R (1990) Fluorometric determination of alkaline phosphatase in fluid dairy products: Collaborative study. J Assoc Off Anal Chem 73:842Google Scholar
  15. 15.
    Davis CE (1998) Fluorometric determination of acid phosphatase in cooked, boneless, nonbreaded broiler breast and thigh meat. J AOAC Int 81:887Google Scholar
  16. 16.
    AACC International (2010) Approved methods of analysis, 11th edn. (online), American Association of Cereal Chemists, St. Paul, MNGoogle Scholar
  17. 17.
    Christen GL, Marshall RT (1984) Selected properties of lipase and protease of Pseudomonas fluorescens 27 produced in 4 media. J Dairy Sci 67:1680CrossRefGoogle Scholar
  18. 18.
    Kim SM, Zayas JF (1991) Comparative quality characteristics of chymosin extracts obtained by ultrasound treatment. J Food Sci 56:406CrossRefGoogle Scholar
  19. 19.
    Reyes J, Cavalieri RP (2003) Biosensors. In: Heldman DR (ed) Encyclopedia of agricultural, food, and biological engineering, Marcel Dekker, New York, pp 119–123Google Scholar
  20. 20.
    Guilbault GG, Lubrano GJ (1972) Enzyme electrode for glucose. Anal Chim Acta 60:254CrossRefGoogle Scholar
  21. 21.
    Borisov SM, Wolbeis OS (2009) Optical biosensors. Chem Rev 108:423Google Scholar
  22. 22.
    Shimizu Y, Morita K (1990) Microhole assay electrode as a glucose sensor. Anal Chem 62:1498CrossRefGoogle Scholar
  23. 23.
    Matsumoto K (1990) Simultaneous determination of multicomponent in food by amperometric FIA with immobilized enzyme reactions in a parallel configuration. In: Schmid RD (ed) Flow injection analysis (FIA) based on enzymes or antibodies, GBF monographs, vol 14. VCH Publishers, New York, pp 193–204Google Scholar

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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.School of Food ScienceWashington State UniversityPullmanUSA

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