Iron Determination in Meat Using Ferrozine Assay
Chromogens are chemicals that react with compounds of interest and form colored products that can be quantified using spectroscopy. Several chromogens that selectively react with minerals are available. In this lab, ferrozine is used to measure ferrous iron in an ashed food sample. The relationship between the absorbance of the chromogen-mineral complex is described by Beer’s Law; in this procedure, a standard curve is generated with a stock iron solution to quantify the mineral in beef samples.
KeywordsAscorbic Acid Ferrous Iron Volumetric Flask Colored Product Meat Sample
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