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Advanced Tests of Sperm Function

  • Joseph P. Alukal
  • Dolores J. LambEmail author
Chapter

Abstract

Sperm function tests assess the presence or absence of factors needed for proper fertilization and initiation of embryogenesis; put differently, these tests measure the ability of a sperm to deliver a normal haploid complement of chromosomes to an oocyte. Advanced tests of sperm function have helped us to gain a tremendous understanding of the physiology of sperm capacitation, acrosome reaction, and sperm-ovum interaction. For this reason alone, an understanding of these tests is still vitally important to the practicing andrologist, reproductive endocrinologist, or embryologist. These tests do accurately predict the likelihood of success with IUI (with or without ovarian hyperstimulation) or IVF. Unfortunately, with the increased use of IVF/ICSI, these tests are less widely used by physicians. Despite this, the usage of these tests to properly identify which couples need to proceed to IVF/ICSI (due to a low likelihood of success with IUI or IVF alone) would represent a significant step forward toward a cost-efficient and health-conscious treatment algorithm for couples with infertility.

Keywords

Sperm function Aneuploidy Capacitation Acrosome reaction DNA damage Sperm penetration assay Hemizona assay 

References

  1. 1.
    Grow DR, Oehninger S, Seltman HJ et al (1994) Sperm morphology as diagnosed by strict criteria: probing the impact of teratozoospermia on fertilization rate and pregnancy outcome in a large in vitro fertilization population. Fertil Steril 62(3):559-567PubMedGoogle Scholar
  2. 2.
    Chohan KR, Griffin JT, Lafromboise M, De Jonge CJ, Carrell DT (2006) Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J Androl 27(1):53-59CrossRefPubMedGoogle Scholar
  3. 3.
    Bungum M, Humaidan P, Axmon A et al (2007) Sperm DNA integrity assessment in prediction of assisted reproduction technology outcome. Hum Reprod 22(1):174-179CrossRefPubMedGoogle Scholar
  4. 4.
    Carrell DT, Liu L, Peterson CM et al (2003) Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl 49(1):49-55CrossRefPubMedGoogle Scholar
  5. 5.
    Zini A, Blumenfeld A, Libman J, Willis J (2005) Beneficial effect of microsurgical varicocelectomy on human sperm DNA integrity. Hum Reprod 20(4):1018-1021CrossRefPubMedGoogle Scholar
  6. 6.
    Egozcue J, Blanco J, Vidal F (1997) Chromosome studies in human sperm nuclei using fluorescence in-situ hybridization (FISH). Hum Reprod Update 3(5):441-452CrossRefPubMedGoogle Scholar
  7. 7.
    Downie SE, Flaherty SP, Matthews CD (1997) Detection of chromosomes and estimation of aneuploidy in human spermatozoa using fluorescence in-situ hybridization. Mol Hum Reprod 3(7):585-598CrossRefPubMedGoogle Scholar
  8. 8.
    Vegetti W, Van Assche E, Frias A et al (2000) Correlation between semen parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men. Hum Reprod 15(2):351-365CrossRefPubMedGoogle Scholar
  9. 9.
    Rubio C, Simon C, Blanco J et al (1999) Implications of sperm chromosome abnormalities in recurrent miscarriage. J Assist Reprod Genet 16(5):253-258CrossRefPubMedGoogle Scholar
  10. 10.
    Jeyendran RS, Van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70(1):219-228CrossRefPubMedGoogle Scholar
  11. 11.
    Tartagni M, Schonauer MM, Cicinelli E et al (2002) Usefulness of the hypo-osmotic swelling test in predicting pregnancy rate and outcome in couples undergoing intrauterine insemination. J Androl 23(4):498-502PubMedGoogle Scholar
  12. 12.
    Biljan MM, Buckett WM, Taylor CT et al (1996) Effect of abnormal hypo-osmotic swelling test on fertilization rate and pregnancy outcome in in vitro fertilization cycles. Fertil Steril 66(3):412-416PubMedGoogle Scholar
  13. 13.
    Jeyendran RS, Van der Ven HH, Zaneveld LJ (1992) The hypoosmotic swelling test: an update. Arch Androl 29(2):105-116CrossRefPubMedGoogle Scholar
  14. 14.
    Mordel N, Dano I, Epstein-Eldan M, Shemesh A, Schenker JG, Laufer N (1993) Novel parameters of human sperm hypoosmotic swelling test and their correlation to standard spermatogram, total motile sperm fraction, and sperm penetration assay. Fertil Steril 59(6):1276-1279PubMedGoogle Scholar
  15. 15.
    Cohlen BJ, te Velde ER, Habbema JD (1999) Postcoital testing. Postcoital test should be performed as routine infertility test. BMJ 318(7189):1008-1009PubMedGoogle Scholar
  16. 16.
    Niederberger CS, Lamb DJ, Glinz M, Lipshultz LI, Scully NF (1993) Tests of sperm function for evaluation of the male: Penetrak and Tru-Trax. Fertil Steril 60(2):319-323PubMedGoogle Scholar
  17. 17.
    Eggert-Kruse W, Gerhard I, Tilgen W, Runnebaum B (1989) Clinical significance of crossed in vitro sperm-cervical mucus penetration test in infertility investigation. Fertil Steril 52(6):1032-1040PubMedGoogle Scholar
  18. 18.
    Eggert-Kruse W, Leinhos G, Gerhard I, Tilgen W, Runnebaum B (1989) Prognostic value of in vitro sperm penetration into hormonally standardized human cervical mucus. Fertil Steril 51(2):317-323PubMedGoogle Scholar
  19. 19.
    Balasch J, Jove I, Ballesca JL et al (1989) Human in vitro fertilization in couples with unexplained infertility and a poor postcoital test. Gynecol Endocrinol 3(4):289-295CrossRefPubMedGoogle Scholar
  20. 20.
    Hull MG, Joyce DN, McLeod FN, Ray BD, McDermott A (1984) Human in-vitro fertilisation, in-vivo sperm penetration of cervical mucus, and unexplained infertility. Lancet 2(8397):245-246CrossRefPubMedGoogle Scholar
  21. 21.
    Hull MG (1992) Infertility treatment: relative effectiveness of conventional and assisted conception methods. Hum Reprod 7(6):785-796PubMedGoogle Scholar
  22. 22.
    Forti G, Krausz C (1998) Clinical review 100: evaluation and treatment of the infertile couple. J Clin Endocrinol Metab 83(12):4177-4188CrossRefPubMedGoogle Scholar
  23. 23.
    Chiu PC, Chung MK, Tsang HY et al (2005) Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa. J Biol Chem 280(27):25580-25589CrossRefPubMedGoogle Scholar
  24. 24.
    Chiu PC, Chung MK, Koistinen R et al (2007) Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding. J Cell Sci 120(Pt 1):33-44PubMedGoogle Scholar
  25. 25.
    Uchida H, Maruyama T, Ohta K et al (2007) Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation. Hum Reprod 22(10):2615-2622CrossRefPubMedGoogle Scholar
  26. 26.
    de Lamirande E, Eiley D, Gagnon C (1993) Inverse relationship between the induction of human sperm capacitation and spontaneous acrosome reaction by various biological fluids and the superoxide scavenging capacity of these fluids. Int J Androl 16(4):258-266CrossRefPubMedGoogle Scholar
  27. 27.
    Kallajoki M, Virtanen I, Suominen J (1986) The fate of acrosomal staining during the acrosome reaction of human spermatozoa as revealed by a monoclonal antibody and PNA-lectin. Int J Androl 9(3):181-194CrossRefPubMedGoogle Scholar
  28. 28.
    Sanchez R, Toepfer-Petersen E, Aitken RJ, Schill WB (1991) A new method for evaluation of the acrosome reaction in viable human spermatozoa. Andrologia 23(3):197-203CrossRefPubMedGoogle Scholar
  29. 29.
    Talbot P, Chacon RS (1981) A triple-stain technique for evaluating normal acrosome reactions of human sperm. J Exp Zool 215(2):201-208CrossRefPubMedGoogle Scholar
  30. 30.
    Perry RL, Naeeni M, Barratt CL, Warren MA, Cooke ID (1995) A time course study of capacitation and the acrosome reaction in human spermatozoa using a revised chlortetracycline pattern classification. Fertil Steril 64(1):150-159PubMedGoogle Scholar
  31. 31.
    Lee MA, Trucco GS, Bechtol KB et al (1987) Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay. Fertil Steril 48(4):649-658PubMedGoogle Scholar
  32. 32.
    Oehninger S, Blackmore P, Morshedi M, Sueldo C, Acosta AA, Alexander NJ (1994) Defective calcium influx and acrosome reaction (spontaneous and progesterone-induced) in spermatozoa of infertile men with severe teratozoospermia. Fertil Steril 61(2):349-354PubMedGoogle Scholar
  33. 33.
    Yovich JM, Edirisinghe WR, Yovich JL (1994) Use of the acrosome reaction to ionophore challenge test in managing patients in an assisted reproduction program: a prospective, double-blind, randomized controlled study. Fertil Steril 61(5):902-910PubMedGoogle Scholar
  34. 34.
    Burkman LJ, Coddington CC, Franken DR, Krugen TF, Rosenwaks Z, Hogen GD (1988) The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 49(4):688-697PubMedGoogle Scholar
  35. 35.
    Overstreet JW, Hembree WC (1976) Penetration of the zona pellucida of nonliving human oocytes by human spermatozoa in vitro. Fertil Steril 27(7):815-831PubMedGoogle Scholar
  36. 36.
    Liu DY, Clarke GN, Lopata A, Johnston WI, Baker HW (1989) A sperm-zona pellucida binding test and in vitro fertilization. Fertil Steril 52(2):281-287PubMedGoogle Scholar
  37. 37.
    Oehninger S, Franken D, Alexander N, Hodgen GD (1992) Hemizona assay and its impact on the identification and treatment of human sperm dysfunctions. Andrologia 24(6):307-321CrossRefPubMedGoogle Scholar
  38. 38.
    Huszar G, Ozenci CC, Cayli S, Zavaczki Z, Hansch E, Vigue L (2003) Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil Steril 79(Suppl 3):1616-1624CrossRefPubMedGoogle Scholar
  39. 39.
    Huszar G, Ozkavukcu S, Jakab A, Celik-Ozenci C, Sati GL, Cayli S (2006) Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr Opin Obstet Gynecol 18(3):260-267CrossRefPubMedGoogle Scholar
  40. 40.
    Huszar G, Jakab A, Sakkas D et al (2007) Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetic aspects. Reprod Biomed Online 14(5):650-663CrossRefPubMedGoogle Scholar
  41. 41.
    Yanagimachi R, Yanagimachi H, Rogers BJ (1976) The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 15(4):471-476CrossRefPubMedGoogle Scholar
  42. 42.
    Aitken RJ, Irvine DS, Wu FC (1991) Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am J Obstet Gynecol 164(2):542-551PubMedGoogle Scholar
  43. 43.
    Margalioth EJ, Feinmesser M, Navot D, Mordel N, Bronson RA (1989) The long-term predictive value of the zona-free hamster ova sperm penetration assay. Fertil Steril 52(3):490-494PubMedGoogle Scholar
  44. 44.
    Perreault SD, Rogers BJ (1982) Capacitation pattern of human spermatozoa. Fertil Steril 38(2):258-260PubMedGoogle Scholar
  45. 45.
    Smith RG, Johnson A, Lamb D, Lipshultz LI (1987) Functional tests of spermatozoa. Sperm penetration assay. Urol Clin North Am 14(3):451-458PubMedGoogle Scholar
  46. 46.
    Johnson A, Bassham B, Lipshultz LI, Lamb DJ (1995) A quality control system for the optimized sperm penetration assay. Fertil Steril 64(4):832-837PubMedGoogle Scholar
  47. 47.
    Gvakharia MO, Lipshultz LI, Lamb DJ (2000) Human sperm microinjection into hamster oocytes: a new tool for training and evaluation of the technical proficiency of intracytoplasmic sperm injection. Fertil Steril 73(2):395-401CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Scott Department of UrologyBaylor College of MedicineHoustonUSA
  2. 2.Laboratory for Male Reproductive Research and Testing, Scott Department of UrologyBaylor College of MedicineHoustonUSA

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