Evaluation of Retinal Degeneration in P27KIP1 Null Mouse

  • Yumi Tokita-Ishikawa
  • Ryosuke Wakusawa
  • Toshiaki Abe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 664)


Purpose: p27kip1 is well-known as a cell cycle inhibitor and also plays an important role for cell differentiation. We hypothesized that if we caused retinal degeneration in a p27(–/–) mouse, then the appropriate method of restoration may be different from that of wild mice and therefore suggest a therapeutic methodology for retinal regeneration.

Methods: Histological and electrophysiological (ERG) examination was performed on p27(–/–) mice retina. We injected N-methy-N-nitrosourea (MNU) to induce retinal degeneration. BrdU was used to identify the dividing cells in the retina.

Results: Thicker retina were observed in the p27(–/–) mice when compared to those of the p27(–/+) mice or wild type mice. Almost all retinal layers were thick and optic nerves were also enlarged. A statistically significant decrease of a and b waves amplitudes of ERG was observed in p27(–/–) mice when compared to those of the other mice. BrdU and nestin positive cells were present at the outer nuclear layer with no difference between p27(–/–) and wild type mice after MNU injection.

Conclusion: p27(–/–) mice showed thicker retina and less retinal function than those of other mice. The MNU-induced retinal degeneration in p27(–/–) mice closely resembled the reaction of the other mice with no retinal regeneration observed in our experimental condition.


Wild Type Mouse Outer Hair Cell BrdU Incorporation Retinal Degeneration Outer Nuclear Layer 
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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yumi Tokita-Ishikawa
    • 1
  • Ryosuke Wakusawa
    • 2
  • Toshiaki Abe
    • 3
  1. 1.Division of Clinical Cell TherapyCenter for Translational and Advanced Animal ResearchMiyagi, SendaiJapan
  2. 2.Department of Ophthalmology and Visual Science, Graduate School of MedicineTohoku UniversityMiyagi, SendaiJapan
  3. 3.Division of Clinical Cell Therapy, School of MedicineTohoku UniversityMiyagi, SendaiJapan

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