Comparative Genomics of Pathogens
The comparative genomics of multiple bacterial strains has become one of the most powerful tools in microbial classification, systematics and evolution. It is also key to the study of the evolutionary dynamics of genomes, gene families, and new functional elements, as well as to the identification of genes that are subject to positive selection and to the identification of lineage-specific genes that are species- and strain-specific. The latter are of special interest as they may play a role in the exclusive ecological adaptations to particular niches on genus-, species-, and strain-specific levels. The focus of this chapter is on new comparative genomics approaches and metagenomics. These can be employed in the molecular identification of bacterial pathogens, in genotyping, and in the detection of virulence genes and other markers.
KeywordsFluorescent Resonance Energy Transfer Bacterial Genome Molecular Beacon Metagenomic Library Multiple Displacement Amplification
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