Pangenomic Reverse Vaccinology
It has become possible to obtain the set of potentially expressed proteins from the DNA sequence and, using a combination of computational and experimental approaches, to select a list of the potential antigens to be tested in animal models. This chapter illustrates how such an innovative process, termed “Reverse Vaccinology” and first applied to the case of Neisseria meningitidis serogroup B, can dramatically increase the efficiency of vaccine development. Authors also demonstrate how new types of information obtained from genetic typing and antigen-based serological typing can support the vaccine development phase as well as the continued clinical surveillance needed upon the introduction of a new vaccine into the field.
KeywordsSingle Nucleotide Polymorphism Ribosomal Binding Site Capsular Polysaccharide Localization Prediction Streptococcus Agalactiae
- Benson DA et al (2008) GenBank. Nucleic Acids Res 36(Database Issue):D25–D30Google Scholar
- Filliol I et al (2006) Global phylogeny of Mycobacterium tuberculosis based on single nucleotide polymorphism (SNP) analysis: insights into tuberculosis evolution, phylogenetic accuracy of other DNA fingerprinting systems, and recommendations for a minimal standard SNP set. J Bacteriol 188(2):759–772CrossRefPubMedGoogle Scholar
- Flicek P et al (2008) Ensembl 2008. Nucleic Acids Res 36(Database issue):D707–D714Google Scholar
- The UniProt Consortium (2008) The universal protein resource (UniProt). Nucleic Acids Res 36(Database issue):D190–D195Google Scholar