Fluorescence-Guided Photodynamic Therapy
The use of fluorescence-guided PDT is thought to be a standard component in every PDT treatment, and in photorejuvenation to ensure treatment efficiency, and to reduce risk of post-treatment phototoxicity. As protoporphyrin IX, in contrast to ALA, fluoresces at 634 nm (at 407 nm excitation), the transformation of ALA can be monitored noninvasively by detecting the skin surface fluorescence. This method has in the past been used in experimental clinical studies to optimize mechanical skin preparation, type of precursor, incubation time, topical delivery mechanism (vehicle) as well as the concentration of ALA.
KeywordsApplication Time Actinic Keratose Fluorescence Level Normal Human Skin Cream Base
- 1.FluoDerm is a handheld device for objective real-time in vivo measuring of fluorescence. 2010. www.Dia-Medico.com. Accessed Feb 2010.
- 5.Kloek J, Akkermans W, Beijersbergen van Henegouwen GM. Derivatives of 5-aminolevulinic acid for photodynamic therapy: enzymatic conversion into protoporphyrin. Photochem Photobiol. 1998;67:150–4.Google Scholar
- 16.Alster TS, Tanzi EL, Welsh CW. Photorejuvenation of Facial skin with 20% 5-Amninolevulinic Acid and Intense Pulsed Light. J Drug Derm. 2005;4:35–8.Google Scholar
- 17.Dover JS, Bhatia AC, Stewart B, Arndt KA. Topical 5-aminolevulinic acid combined with intense pulsed light in the treatment of photoaging. Arch Dermatol 2005;141(10):1247–52.Google Scholar
- 19.Gold MH, Bradshaw VL, Boring MM, Bridges TM, Biron JA. Split-face comparison of photodynamic therapy with 5-aminolevulinic acid and intense pulsed light versus intense pulsed light alone for photodamage. Dermatol Surg. 2006;32(6):795–801; discussion 801–3.Google Scholar
- 20.Laurent A, Mistretta F, Bottigioli D, Dahel K, Goujon C, Nicolas JF, Hennino A, Laurent PE. Echographic measurement of skin thickness in adults by high frequency ultrasound to assess the appropriate microneedle length for intradermal delivery of Vaccine 2007;21:25(34):6423–30.Google Scholar