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Spectroscopic Characterization of Plasma – Chemically Functionalized and Fluorophore-Labeled Polymer Surfaces

  • Katrin Hoffmann
  • Renate Mix
  • Joerg F. Friedrich
  • Ute Resch-Genger
Part of the Reviews in Fluorescence 2008 book series (RFLU, volume 2008)

Abstract

The potential of spectrofluorometry and fluorescence microscopy for the characterization and quantification of different functionalities like OH and NH2 groups at plasma-chemically modified polymer surfaces is assessed using traditional reactive dyes such as dansyl derivatives and a sophisticated VIS-excitable chromogenic and fluorogenic pyrylium label showing binding-induced spectral and intensity changes in absorption and emission. Aiming at an improved fluorometric surface analysis, based upon these measurements, several sources of uncertainty inherent to fluorescence measurements are illustrated ranging from environment-dependent dye absorption and emission features over spectral correction and nonspecific adsorption to the critical influence of label choice on the measured background. Solutions to these drawbacks are given thereby underlining the potential of fluorometry for surface analysis.

Keywords

Water Contact Angle Surface Functionality Fluorescence Quantum Yield Emission Polarizer Total Internal Reflection Fluorescence Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Katrin Hoffmann
    • 1
  • Renate Mix
    • 1
  • Joerg F. Friedrich
    • 1
  • Ute Resch-Genger
    • 2
  1. 1.Federal Institute for Materials Research and Testing (BAM)BerlinGermany
  2. 2.Working Group Fluorescence Spectroscopy, Department IBAM Federal Institute for Materials Research and TestingBerlinGermany

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