Imaging Protein Interactions in Living Cells Using the Fluorescent Proteins

  • Richard N. Day
  • Ammasi Periasamy
  • Ignacio Demarco
Part of the Reviews in Fluorescence 2008 book series (RFLU, volume 2008)


Over the past decade, the visible fluorescent proteins (VFPs) have become an essential tool for the study of cell biology and physiology. The goal of this chapter is to describe the characteristics of the FPs and discuss the limits of their use as probes for noninvasive imaging of protein behavior in living cells. We also described live-cell imaging techniques used to monitor the subcellular dynamics and interactions of proteins in living cells. We then focused on the method of fluorescence resonance energy transfer (FRET) microscopy to quantify the interactions of proteins inside living cells and consider some of the factors that limit this approach.


Fluorescence Resonance Energy Transfer Fluorescence Recovery After Photobleaching Fluorescence Lifetime Imaging Microscopy Fluorescence Resonance Energy Transfer Efficiency Fluorescence Resonance Energy Transfer Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Richard N. Day
    • 1
  • Ammasi Periasamy
    • 2
  • Ignacio Demarco
    • 3
  1. 1.Departments of Medicine and Cell BiologyUniversity of Virginia Health ServicesCharlottesvilleUSA
  2. 2.Keck Center for Cellular Imaging, Department of BiologyUniversity of VirginiaCharlottesvilleUSA
  3. 3.Department of Cell BiologyUniversity of Virginia Health ServicesCharlottesvilleUSA

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