Intracortical Microcirculatory Change Induced by Anesthesia in Rat Somatosensory Cortex

  • Kazuto Masamoto
  • Takayuki Obata
  • Iwao Kanno
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 662)


The present study aimed to characterize microcirculatory responses to anesthesia in brain tissue. With multi-photon excitation fluorescence microscopy, intra-cortical capillary dimension and red blood cell (RBC) flow were successfully visualized up to a depth of ∼0.6 mm from the cortical surface in rats anesthetized with either isoflurane or α-chloralose. We observed that the diameter of the major cerebral artery was ∼100 µm under isoflurane, but ∼75 µm under α-chloralose. The capillary diameter was observed to be larger under α-chloralose than isoflurane: 5.1 ± 1.2 µm vs. 4.8 ± 1.1 µm, respectively. A significant difference in the mean RBC speed measured in single capillaries was observed: 0.4 ± 0.4 mm/s under α-chloralose vs. 1.5 ± 0.4 mm/s under isoflurane. In agreement with these observations, arterio-venous transit-time and laser-Doppler flowmetry consistently showed a significant reduction of the RBC and plasma blood speed under α-chloralose relative to isoflurane. These findings may indicate that local blood flow regulatory mechanisms exist at the capillary level for the balance of oxygen supply and demand induced by anesthesia in the brain tissue.


Cerebral Blood Flow Cortical Surface Single Capillary Baseline Cerebral Blood Flow Tissue Oxygen Level 
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This work was partially supported by KAKENHI (#19800065).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Molecular Imaging Center, National Institute of Radiological SciencesChibaJapan

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