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Arteriolar Vasomotor Control and Contractile Performance During Fatiguing Tetanic Contractions in Rat Skeletal Muscle: Role of Sympathetic System

  • Tadakatsu Inagaki
  • Takashi Sonobe
  • David C. Poole
  • Yutaka Kano
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 662)

Abstract

Using a fatiguing stimulation protocol designed specifically to enhance sympathetically-mediated vasoconstrictor tone, we explored the temporal profile of the evoked vasoconstrictor response, evaluated the presence of sympatholysis, and assessed the role of α1-adrenergic receptor-mediated vasoconstriction on muscle performance. Spinotrapezius muscles of Wistar rats were exteriorized and stimulated tetanically (100 Hz, 4–7 V, stimulus duration 700 ms) every 3 s for 2.5 min under control and prazosin (1 μM) superfused conditions. The extent and time course of diameter changes in arterioles (2 A) and venules (2 V) were determined after each of 10 discrete sets of muscle stimulation at 5-min intervals. A significant decrease of luminal diameter was observed in arterioles after tetanic contractions at 8–10 sets (8 sets: −34.4%, 9 sets: −39.4%, 10 sets: −38.6% vs pre-contraction at each set, p < 0.01). Prazosin significantly reduced but did not abolish the contraction-induced vasoconstriction. In both conditions, there was no reduction of venules diameter observed. Tetanic contractions force at the final 10th set was significantly decreased to 29.3 ± 11.9% from pre-fatigue conditions, while tetanic contractions with prazosin force production was maintained at 70.4 ± 14.2% at the 10th set. We conclude that in sequential bouts of contractions there was a progressively greater degree of arteriolar (but not venular) vasoconstriction which was attenuated substantially by prazosin.

Keywords

Muscle Fatigue Muscle Sympathetic Nerve Activity Muscle Blood Flow Tetanic Contraction Tetanic Force 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Tadakatsu Inagaki
    • 1
  • Takashi Sonobe
    • 1
  • David C. Poole
    • 2
    • 3
  • Yutaka Kano
    • 1
  1. 1.Departments of Applied Physics and ChemistryUniversity of Electro-CommunicationsChohuJapan
  2. 2.Departments of Anatomy, Physiology and KinesiologyKansas State UniversityManhattanUSA
  3. 3.School of Sports and Health SciencesUniversity of ExeterExeterUK

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