Skip to main content

Force Microscopy on Membrane Patches

A Perspective

  • Chapter
Single-Channel Recording

Abstract

Force microscopy, originally named atomic force microscopy by the inventors (Binnig et al., 1986), is a new type of measuring instrument in the nanometer range, down to the size of single atoms, developed after the invention of the scanning tunneling microscope (Binnig et al., 1982). These microscopes represent not only new types of imaging instruments, hence the term “microscopes,” but, more importantly, a new technology called scanning probe techniques (SPT). With these methods, one can measure and manipulate in the nanometer range in any environment, opening up a new field of interdisciplinary experimental science. The common features of these microscopes are a measurable and strongly distance-dependent parameter, a probe small enough for the desired spatial resolution, and a mechanism to scan over a surface with the necessary stability.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 299.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 379.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Binnig, G., Rohrer, H., Gerber, C., and Weibel, E., 1982, Surface study by scanning tunneling microscopy, Phys. Rev. Lett 49: 57–60.

    Article  Google Scholar 

  • Binnig, G., Quate, C. F., and Gerber, C., 1986, Atomic force microscope, Phys. Rev. Lett 56: 930–933.

    Article  PubMed  Google Scholar 

  • Bryant, A., Smith, D. P. E., Ohnesorge, E, Weisenhorn, A. J., Heyn, S. P., Drake, B., Prater, C. B., Gould, S. A., Hansma, P. A., and Gaub, H., and Quate, C. F., 1986, Appl. Phys. Lett 48: 832–834.

    Google Scholar 

  • Egger, M., Ohnesorge, F., Weisenhorn, A. J., Heyn, S. P., Drake, B., Prater, C. B., Gould, S. A.

    Google Scholar 

  • Hansma, P. A., and Gaub, H., 1990, Wet lipid—protein membranes imaged at submolecular resolution by atomic force microscopy, J. Struct. Biol 103: 89–94.

    Article  Google Scholar 

  • Fuchs, H., Schrepp, W., and Rohrer, H., 1987, STM investigations of Langmuir—Blodgett films, Surf. Sci 181: 391–393.

    Article  CAS  Google Scholar 

  • Guckenberger, R., Weigrabe, W., Hillebrand, A., Hartmann, T., Wang, Z., and Baumeister, W., 1989, Scanning tunneling microscopy of a hydrated bacterial surface protein, Ultramicroscopy 31: 327–331.

    Article  CAS  Google Scholar 

  • Häberle, W., Hörber, J. K. H., and Binnig, G., 1989, Force microscopy on living cells, J. Vac. Sci.Technol B9: 1210–1212.

    Google Scholar 

  • Hansma, P. K., Drake, B., Marti, O., Gould, S. A. C., and Prater, C. B., 1989, The scanning ion conductance microscope, Science 243: 641–643.

    Article  PubMed  CAS  Google Scholar 

  • Hong, K., and Driscoll, M., 1994, A transmembrane domain of the putative channel subunit MEC-4 influences mechanotransduction and neurodegeneration in C. elegans, Nature 367: 470–474.

    Article  PubMed  CAS  Google Scholar 

  • Hörber J. K. H., Lang, C. A., Hänsch, T. W., Heckl, W. M., and Möhwald, H., 1988, Scanning tunneling microscopy of lipid films and embedded biomolecules, Chem. Phys. Leu 145: 151–154.

    Article  Google Scholar 

  • Hörber, J. K. H., Schuler, F. M., Witzemann, W., Schröder, K. H., and Müller, H., 1991, Imaging of cell membrane proteins with a scanning tunneling microscope, J. Vac. Sci. Technol B9: 1214–1218.

    Article  Google Scholar 

  • Hörber, J. K. H., Häberle, W., Ohnesorge, F., Binnig, G., Liebich, H. G., Czerny, C. P., Mahnel, H., and Mayr, A., 1992, Investigation of living cells in the nanometer regime with the scanning force microscope, Scan. Microsc 6: 919–929.

    Google Scholar 

  • Huang, M., and Chalfie, M., 1994, Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans, Nature 367: 467–470.

    Article  PubMed  CAS  Google Scholar 

  • Hudspeth, A. J., and Gillespie, P. G., 1994, Pulling springs to tune transduction: Adaption by hair cells, Neuron 12: 1–9.

    Article  PubMed  CAS  Google Scholar 

  • Israelachvili, J. N., 1992, Intermolecular and Surface Forces, 2nd. ed., Academic Press, San Diego. Jericho, M. H., Blackford, B. D., Dahn, D. C., Frame, C., and Maclean, D., 1990, Scanning tunneling microscopy imaging of uncoated biological material, J. Vac. Sci. Technol 88: 661–666.

    Google Scholar 

  • Martin, Y., and Wickramasinghe, H. K., 1988, High resolution capacitance measurement and potentiometry by force microscopy, Appl. Phys. Lett 52: 1103.

    Article  Google Scholar 

  • Matey, J. R., and Blanc, J., 1985, Scanning capacitance microscopy J. Appl. Phys. 57:1437–1439. Methfessel, C., Witzemann, V., Takahashi, T., Mishima, M., Numa, S., and Sakmann, B., 1986, Patch clamp measurements on Xenopus laevis oocytes: Currents through endogenous channels and implanted acetylcholine receptor and sodium channels, Pflügers Arch. 407: 577–588.

    Google Scholar 

  • Meyer, E., Howald, L., Overney, R. M., Heinzelmann, H., Frommer, J., Guntherodt, H.-J., Wagner, T., Schier, H., and Roth, S., 1989, Molecular-resolution images of Langmuir—Blodgett films using atomic force microscopy, Nature 349: 398–399.

    Article  Google Scholar 

  • Meyer, G., and Am, N. M., 1988, Novel optical approach to atomic force microscopy, Appl. Phys. Lett 53: 1045–1047.

    Article  Google Scholar 

  • Pohl, D. W., Fischer, U. C., and Dtirig, U. T., 1988, Scanning near-field optical microscopy (SNOM), J. Microsc 152: 853–861.

    Article  Google Scholar 

  • Sâenz, J. J., Garcia, N., Grutter, E, Meyer, E., Heinzelmann, H., Wiesendanger, R., Rosenthaler, L., Hidber, H. R., and Guntherodt, H.-J., 1987, Observation of magnetic forces by the atomic force microscope, J. Appl. Phys 62: 4293–4295.

    Article  Google Scholar 

  • Smith, D. P. E., Bryant, A., Quade, C. F., Rabe, J. P., Berger, Ch., and Swalen, J. D., 1987, Images of a lipid bilayer at molecular resolution by scanning tunneling microscopy, Proc. Natl. Acad. Sci. U.S.A 84: 969–972.

    Article  PubMed  CAS  Google Scholar 

  • Sukharev, S. I., Blount, P., Martinac, B., Blattner, F. R., and Kung, C., 1994, A large-conductance mechanosensitive channel in E. coli encoded by mscL alone, Nature 368: 265–268.

    Article  PubMed  CAS  Google Scholar 

  • Williams, C. C., and Wickramasinghe, H. K., 1988, Thermal and photo thermal imaging on a sub 100 nm scale, Proc. SPIE 897: 129.

    Article  CAS  Google Scholar 

  • Worcester, D. L., Miller, R. G., and Bryant, P. J., 1988, Atomic force microscopy of purple membranes, J. Microsc 152: 817–821.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer Science+Business Media New York

About this chapter

Cite this chapter

Hörber, J.K.H., Mosbacher, J., Häberle, W. (1995). Force Microscopy on Membrane Patches. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-1229-9_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4419-1229-9_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-1230-5

  • Online ISBN: 978-1-4419-1229-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics