MicroRNA Profiling and Its Application in Drug Discovery in Pancreatic Cancer
MicroRNAs (miRNAs) are single-stranded small RNA molecules of 21–23 nucleotides in length, involved in regulation of gene expression. An emerging number of studies show that miRNAs play a vital role in important signaling pathways in human oncogenesis. miRNAs can be utilized as potential biomarkers for cancer diagnosis and also as candidates in cancer treatment drug development. Pancreatic cancer is a lethal disease and is the fourth leading cause of cancer-related deaths in the United States. One key factor that can make an impact is earlier diagnosis of the disease, when surgery could offer patients a chance of cure. Use of miRNAs for early detection and drug development offers a unique opportunity. Identification and assessment of miRNA functionality that are differentially expressed in pancreatic cancer originates from methods for miRNA profiling such as miRNA microarray, in situ hybridization, and various computational algorithms applied for data analysis. This chapter discusses the various profiling methods applied to the identification of differentially expressed miRNAs in pancreatic cancer, which can applied towards drug development for pancreatic cancer.
KeywordsPancreatic Cancer miRNA Expression Pancreatic Adenocarcinoma Pancreatic Cancer Patient miRNA Profile
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