Microbiological Spoilage of Spices, Nuts, Cocoa, and Coffee

  • Joan M. Pinkas
  • Karen Battista
  • Theodora Morille-Hinds
Part of the Food Microbiology and Food Safety book series (FMFS)


Spices, nuts, cocoa, and coffee are raw materials that may be used alone or as ingredients in the manufacture of processed food products. The control of microbiological spoilage of these raw materials at the ingredient stage will enable the food processor to better assure the production of high-quality foods with an acceptable shelf life. While this chapter is limited to four materials, many of the spoilage control procedures recommended can also be applied to other raw materials of a similar nature.


Coffee Bean Black Pepper Mold Growth Cocoa Bean Codex Alimentarius Commission 


  1. Adebajo, L. O., & Diyaolu, S. A. (2003). Mycology and spoilage of retail cashews. African Journal of Biotechnology, 2(10), 369–373.Google Scholar
  2. Akerstrand, K. (1992). Mould counts and mycoflora in samples of spices as influenced by medium and plating technique. In R. A. Samson, A. D. Hocking, J. I. Pitt, & A. D. King (Eds.), Modern methods in food mycology (pp. 141–143). New York: Elsevier.Google Scholar
  3. American Spice Trade Association. (2001a). Spice statistics 2000. Washington, DC: American Spice Trade Association.Google Scholar
  4. American Spice Trade Association. (2001b). ASTA cleanliness specifications for spices, seeds, and herbs (Revised April 28, 1999). Washington, DC: American Spice Trade Association.Google Scholar
  5. American Whole Health. (2000). http://www.wholehealthmd.com
  6. Anonymous. (2006). About cashews. http://dacnet.nic.in/cashewcocoa/tech.htm
  7. Arunga, R. O. (1982). Coffee. In A. H. Rose (Ed.), Fermented foods, economic microbiology (Vol. 7, pp. 259–274). London: Academic Press.Google Scholar
  8. Avallone, S., Guiraud, J. P., Guyot, B., Olguin, E., & Brillouet, J. M. (2001). Fate of mucilage cell wall polysaccharides during coffee fermentation. Journal of Agricultural and Food Chemistry, 49, 5555–5559.CrossRefGoogle Scholar
  9. Avallone, S., Brillouet, J. M., Guyot, B., Olguin, E., & Guiraud, J. P. (2002). Involvement of pectolytic microorganisms in coffee fermentation. International Journal of Food Science and Technology, 37, 191–198.CrossRefGoogle Scholar
  10. Aziz, N. H., Youssef, Y. A., El-Fouly, M. Z., & Moussa, L. A. (1998). Contamination of some common medicinal plant samples and spices by fungi and their mycotoxins. Botanical Bulletin of Academia Sinica, 39, 279–285.Google Scholar
  11. Baxter, R., & Holzapfel, W. (1982). A microbial investigation of selected spices, herbs, and additives in South Africa. Journal of Food Science, 47, 570–578.CrossRefGoogle Scholar
  12. Beuchat, L. (Ed.). (1987). Food and beverage mycology (2nd ed.). Relationship of water activity to fungal growth (pp. 51–88); Field and storage fungi ( pp. 211–231). Van Nostrand Reinhold, AVI, New York.Google Scholar
  13. Beuchat, L. (2002). IFT Fundamentals of Water Activity Short Course, Water Activity and Microbial Stability. http://www.wateractivity.org
  14. Beuchat, L. R., Frandberg, E., Deak, T., Alzamora, S. M., Chen, J., Guerrero, A. S., et al. (2001). Performance of mycological media in enumerating desiccated food spoilage yeasts: An interlaboratory study. International Journal of Food Microbiology, 70, 89–96.CrossRefGoogle Scholar
  15. Candlish, A. A., Pearson, S. M., Aidoo, K. E., Smith, J. E., Kelly, B., & Irvine, H. (2001). A survey of ethnic foods for microbial quality and aflatoxin content. Food Additives Contamination, 18, 129–136.CrossRefGoogle Scholar
  16. Cardoza, Y., Lait, C., Schmelz, E., Huang, J., & Tumlison, J. (2003). Fungus – Induced biochemical changes in peanut plants and their effect on development of beet armyworm. Environmental Entomology, 32, 220–228.CrossRefGoogle Scholar
  17. Castelein, J., & Verachtert, H. (1981). Coffee fermentation. In H. J. Rehm & G. Reed, (Eds.), Biotechnology: A comprehensive treatise in 8 volumes (Vol. 5, pp. 587–598). Weinheim, Germany: Verlag Chemie.Google Scholar
  18. Chatt, E. M. (1953). Cocoa cultivation, processing, analysis. New York: Wiley/Interscience.Google Scholar
  19. Chittenden, F. J. (Ed.). (1951). Royal horticultural society dictionary of gardening (p. 2098). Oxford: Clarendon Press.Google Scholar
  20. Chourasia, H. K. (1995). Mycobiota and mycotoxins in herbal drugs of Indian pharmaceutical industries. Mycological Research, 99(6), 697–703.CrossRefGoogle Scholar
  21. Christian, J. H. B. (2000). Drying and reduction of water activity. In B. M. Lund, T. C. Baird-Parker, & G. W. Gould (Eds.), The microbiological safety and quality of food (pp. 153–154). Maryland: Aspen.Google Scholar
  22. Codex Alimentarius Commission, RCP 6. (1972). Recommended international code of hygienic practice for tree nuts. Google Scholar
  23. Codex Alimentarius Commission, RCP 42. (1995). Code of hygienic practice for spices and dried aromatic plants. Google Scholar
  24. Coleman, R. J., Lenney, J. F., Coscia, A.T., & Dicarlo, F. J. (1955). Pectic acid from the mucilage of coffee cherries. Archives of Biochemistry and Biophysics, 59, 157–165.CrossRefGoogle Scholar
  25. Cunningham, S. (1989). The effect of roasting and other processes on almond quality. Manufacturing Confectioner,, 67–70Google Scholar
  26. Downes, F. P., & Ito, K. (Eds.). (2001). Compendium of methods for the microbiological examination of foods (4th ed.). Washington, DC: American Public Health Association.Google Scholar
  27. Entomological Society of America. (2004). http://esa.confex.com
  28. Farkas, J., & Andrassy, E. (1988). Comparative analysis of spices decontaminated by ethylene oxide or gamma radiation. Acta Aliment. 17, 77–94.Google Scholar
  29. Food and Agriculture Organization of the United Nations. (2003). Manual on the application of the Haccp system in mycotoxin prevention and control, Food and Agricultural Organization of the United Nations Food and Nutrition Papers, 73, http://www.fao.org//docrep/005/y1390e/y1390e04.htm
  30. Forsythe, W. G. C., & Quesnel, V. (1963). The mechanism of cocoa curing. Advanced Enzymology, 25, 457–492.Google Scholar
  31. Franco, C. M. (1958). Influence of temperature on growth of coffee plant (Bulletin No. 16, pp. 1–24). New York: IBEC Research Institute.Google Scholar
  32. Frank, H. A., & De la Cruz, A. S. (1964). Role of incidental microflora in natural decomposition of mucilage layer in Kona coffee cherries. Journal of Food Science, 29, 850–853.CrossRefGoogle Scholar
  33. Gaetan, S., & Madia, M. (1993). The presence of cumin (Cuminum cyminumL.) wilt caused by Fusarium oxysporum Schl. F.sp.cumini in Argentina. Boletin de Sanidad Vegetal, Plagas, 19, 503–507.Google Scholar
  34. Garcia, S., Iracheta, F., Galvan, F., & Heredia, N. (2001). Microbiological survey of retail herbs and spices from Mexican markets. Journal of Food Protection, 64(1), 99–103.Google Scholar
  35. Gibson, A., & Butty, M. (1975). Over fermented coffee beans (“stinkers”): a method for their detection and elimination. In Proceedings of the 7th International Scientific Colloquium on Coffee (pp. 141–152). Hamburg, Germany, Paris: Association Scientifique Internationale du Café.Google Scholar
  36. Guirado Moya, M. L., Aguilar, M. L., Blanco, R., Kenig, A., Gomez, J., & Tello, J. C. (2004). Fusarium wilt on sweet basil: Cause and sources in Southeastern Spain. Phytoparasitica, 32, 395–401.CrossRefGoogle Scholar
  37. Hansen, A. P. (1975). Understanding the microbiological deterioration of cocoa. Candy Snack Industry, 140, 44–47.Google Scholar
  38. Hansen, A. P., & Welty, R. E. (1970). Microflora of raw cacao beans. Mycopathology Mycology Applied, 44, 309–316.CrossRefGoogle Scholar
  39. Heredia, N., Galvan, F., Garcia, S., & Iracheta, F. (2001). Microbiological survey of retail herbs and spices from Mexican markets. Journal of Food Protection, 64(1), 99–103.Google Scholar
  40. Hocking, A. (1988). Moulds and yeasts associated with foods of reduced water activity: Ecological interactions (pp. 57–71). International Food and Information Services, Food Science and Technology Abstracts, Berkshire, United Kingdom.Google Scholar
  41. Hocking, A. D. (1992). Collaborative study on media for enumeration of xerophilic fungi. In R. A. Samson, A. D. Hocking, J. I. Pitt, & A. D. King (Eds.), Modern methods in food mycology (pp. 121–125). New York: Elsevier.Google Scholar
  42. Holscher, H., & Steinhart, H. (1995). Aroma compounds in green coffee. In G. Charalambous (Ed.), Food flavors – generation, analysis and process influence (pp. 785–803). Amsterdam: Elsevier Science.CrossRefGoogle Scholar
  43. Horn, B. (2005). Colonization of wounded peanut seeds by soil fungi: Selectivity for species from Aspergillus section flavus. The Mycological Society of America, 97, 202–217.CrossRefGoogle Scholar
  44. Hsieh, R.C., Johnson, S. M., & Dudek, D. H. (1989). Process for sterilization of spices and leafy herbs. U.S. Patent 4,844,933.Google Scholar
  45. International Commission on Microbiological Specifications for Foods. (1980). Microbial ecology of foods, vol. 2 food commodities (p. 745). New York: Academic Press.Google Scholar
  46. International Commission on Microbiological Specifications for Foods. (1986). Micro-organisms in foods: Sampling for microbiological analysis: Principles and specific applications (2nd ed.). Toronto: University of Toronto Press.Google Scholar
  47. International Commission on Microbiological Specifications for Foods. (1998). Microbial ecology of foods, vol. 6. microorganisms in food (pp. 356–378). London: Blackie Academic & Professional.Google Scholar
  48. International Programme on Chemical Safety. (1979). World Health Organization, Environmental Health Criteria 11, Mycotoxins, (p. 8), http://www.inchem.org/documents/ehc/ehc/ehco11.htm
  49. Jackels, S. C., & Jackels C. F. (2005). Characterization of the coffee mucilage fermentation process using chemical indicators: A field study in Nicaragua. Journal of Food Science, 70, C321–C325.CrossRefGoogle Scholar
  50. Joshi, M. S., & Raut, S. P. (1994). Fusarial wilt of clove in the nursery. Current research – University of Agricultural Sciences (Bangalore), 23(1/2), 20.Google Scholar
  51. Julseth, R., & Deibel, R. (1974). Microbial profile of selected spices and herbs at import. Journal of Milk Food Technology, 37, 414–419.Google Scholar
  52. Kavanagh, T. E., Reineccius, G. A., Keeney, P. G., & Weissberger, W. (1970). Mold induced changes in cocoa lipids. Journal of American Oil Chemists Society, 47, 344–346.CrossRefGoogle Scholar
  53. Kinderlerer, J., & Phillips-Jones, M. (1992). Mycology and spoilage of hazelnuts. Food research centre and department of biomedical sciences. Modern methods in food mycology procedures 2 nd international workshop (pp. 133–139).Google Scholar
  54. Kiss, I., & Farkas, J. (1988). Irradiation as a method for decontamination of spices. Food Reviews International, 4, 77–92.CrossRefGoogle Scholar
  55. Kithu, C. J. (2002). Spicing up trade. Times Agriculture Journal, Nov/Dec, http://www.etagriculture.com/nov_dec2002/cover.html
  56. Lehrian, D. W., & Patterson, G. R. (1983). Cocoa fermentation. In G. Reed (Ed.), Biotechnology, a comprehensive treatise (Vol. 5). Switzerland: Verlag Chemie.Google Scholar
  57. Liang, T., Meng, Q., & Ji, F. (1996). Prediction of macadamia nut spoilage for harvest decision making. Journal of Agricultural Engineering Research, 63, 237–242.CrossRefGoogle Scholar
  58. Liardon, R., Braendlin, N., & Spadone, J. C. (1992). Biogenesis of Rio flavor impact compound 2,4,6, trichloroanisole. Proceedings 14th International Conference Coffee Science, San Francisco, 14–19 July 1991.Paris: Association Science.Google Scholar
  59. Mahoney, N., & Molyneux, R. (1998). Contamination of tree nuts by aflatoxigenic fungi: Aflatoxin content of closed – shell pistachios. Journal of Food Chemistry, 46, 1906–1909.CrossRefGoogle Scholar
  60. Mandeel, Q. A. (2005). Fungal contamination of some imported spices. Mycopathologia, 159, 291–298.CrossRefGoogle Scholar
  61. Maravalhas, N. (1966). The effect of “dancing” on the quality of fermented cocoa. Tropical Agricultural (Trin.), 43, 351–354.Google Scholar
  62. Misra, N. (1981). Influence of temperature and relative humidity on fungal flora of some spices in storage. Zeitschriftfur Lebensmittel-Untersuchung Und-Forschung, 172, 30–31.CrossRefGoogle Scholar
  63. Naseema, A., & Sulochana, K. (1994). A new leaf spot of nutmeg. Indian Phytopathology, 47, 439.Google Scholar
  64. Northmore, J. M. (1969). Over fermented beans and stinkers as defectives of arabica coffee. A SIX. 4th Coll., Amsterdam.Google Scholar
  65. Ostovar, K., & Keeney, P. G. (1973). Isolation and characterization of microorganisms involved in the fermentation of Trinidad’s cocoa beans. Journal of Food Science, 38, 11–17.CrossRefGoogle Scholar
  66. Ozdemir, M., & Devres, O. (1999). Turkish hazelnuts: Properties and effects of microbiological and chemical changes on quality. Istanbul, Turkey: Food Science and Technology Research Institute.Google Scholar
  67. Pitt, J. I.,& Hocking, A. D. (1999). Spoilage of stored, processed and preserved foods. Fungi and food spoilage (pp. 494–497). Aspen Publishers, Inc.Google Scholar
  68. Posnette, A. F., & Entwistle, H. M. (1958). The pollination of cocoa flowers. Rep. Cocoa Conf., London 1957, 66–68.Google Scholar
  69. Pruthi, J. S. (1980). Spices and condiments: Chemistry, microbiology, technology. Advances in food research supplement 4. New York: Academic Press.Google Scholar
  70. Rohan, T. A. (1963). Processing of raw cocoa. 1. Small scale fermentation. Journal of the Science of Food and Agriculture, 9, 104–111.CrossRefGoogle Scholar
  71. Roelofsen, P. A. (1958). Fermentation, drying, and storage of cacao bean. Advanced Food Research, 8, 225–296.CrossRefGoogle Scholar
  72. Schwan, R. F., Rose, A. H., & Board, R. G. (1995). Microbial fermentation of cocoa beans, with emphasis on enzymatic degradation of the pulp. Journal of Applied Bacteriology Symposium, Suppl. 79, 96S–107S.Google Scholar
  73. Sivetz, M., & Desrosier, N. W. (1979). Coffee technology. Westport, CT: AVI Publishing Co.Google Scholar
  74. Spadone, J. C., Takeoka, G., & Liardon, R. (1990). Analytical investigation of Rio o-flavor in green coffee. Journal of Agricultural and Food Chemistry, 38, 226–233.CrossRefGoogle Scholar
  75. Sze-Tao, K., Schrimpf, J., Teuber, S., Roux, K., & Sathe, S. (2001). Effects of processing and storage on walnut (Julglans regiaL.) tannins. Journal of the Science of Food Agriculture, 81, 1215–1222.CrossRefGoogle Scholar
  76. Tainter, D. R., & Grenis, A. T. (2001). Spices and seasonings: A food technology handbook. New York: Wiley-VCH.Google Scholar
  77. The Bugwood Network. (1995). http://www.budwood.org
  78. Transport Information Service. (2005). Cargo Loss Prevention Information from German Marine Insurers, http://www.tis-gdv.de/tis_search/result_e.jsp
  79. Tsror, L., Erlich, O., Amitai, S., & Hazanovsky, M. (1998). Verticillium wilt of paprika caused by a highly virulent isolate of Verticillium dahliae. Plant Diseases, 82, 437–439.CrossRefGoogle Scholar
  80. U.S. Environmental Protection Agency. (2005). Ethylene oxide; Tolerances for residues. 40 CFR 180.151. Washington, DC: U.S. Government Printing Office.Google Scholar
  81. U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition. (1998a).  Chapter 8, Spices, condiments, flavors, and crude drugs. In FDA Technical Bulletin Number 5, Macroanalytical Procedures Manual Electronic Version 1998, http://www.cfsan.fda.gov/~dms/mpm-5.html
  82. U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition. (1998b).  Chapter 10, Nuts and nut products. In FDA Technical Bulletin Number 5, Macroanalytical Procedures Manual Electronic Version 1998, http://www.cfsan.fda.gov/~dms/mpm-5.html
  83. U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition. (2001). Bacteriological Analytical Manual Online, January, 2001, http://www.cfsan.fda.gov/~ebam-toc.html
  84. U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition. (2005). The Food Defect Action Levels, September, 2005, http://www.cfsan.fda.gov/~dms/dalbook.html
  85. U.S. Food and Drug Administration, Department of Health and Human Services (2006). Ionizing radiation for the treatment of food. 21 CFR 179.26. Washington, DC: U.S. Government Printing Office.Google Scholar
  86. Van Pee, W., & Castelein, J. M. (1972). The yeast flora of fermenting robusta coffee. East African Agriculture Journal, 36, 308–311.Google Scholar
  87. Weber, F. E. (1980). Controlling microorganisms in spices. Cereal Foods World, 25, 319–321.Google Scholar
  88. Wood, G. A. R. (1988). From harvest to store. In G. Wrigley (Ed.), Cocoa (5th ed., pp. 478–483). New York: Longman.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joan M. Pinkas
    • 1
  • Karen Battista
    • 2
  • Theodora Morille-Hinds
    • 3
  1. 1.McCormick & Co., Inc.Hunt ValleyUSA
  2. 2.Kraft FoodsEast HanoverQuery
  3. 3.Kraft FoodsTarrytownUSA

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