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Nanodiamonds pp 235-247 | Cite as

Biomechanics of Single Cells and Cell Populations

  • Michael A. Teitell
  • Sheraz Kalim
  • Joanna Schmit
  • Jason Reed
Chapter

Abstract

Cells form the basic unit of life. Their health and activities can be quantified by a multitude of biochemical and biophysical techniques that measure responses to external or internal stimuli. Many experimental approaches attempt to integrate molecular mechanisms with changes in the mechanical properties of cells, such as visocoelasticity and compliance, to link cell function with structure. An emerging view of cellular heterogeneity is that even within homogenous cell populations, individual cells may exhibit unique behavioral characteristics that deviate significantly from the population average. Here, several approaches for quantifying biophysical cellular responses are briefly reviewed and linked to specific underlying molecular mechanisms. We succinctly describe each approach and then elaborate on a new interferometer-based method for higher-throughput biophysical analysis of single cells within populations.

Keywords

Atomic Force Microscopy Cancer Stem Cell Atomic Force Microscopy Study Cell Rigidity Micropipette Aspiration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Work on optical profilometry in the Teitell lab is generously supported by the UC Discovery/Abraxis Biosciences Biotechnology Award Bio07-10663. The authors thank Kayvan Niazi and Shahrooz Rabizadeh (Abraxis Biosciences) for encouragement and insightful discussions, and Daphne Weihs (Technion University), for critical reading and comments on the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Michael A. Teitell
    • 1
    • 2
    • 3
  • Sheraz Kalim
    • 1
  • Joanna Schmit
    • 4
  • Jason Reed
    • 2
    • 5
  1. 1.Department of Pathology and Laboratory MedicineUCLALos AngelesUSA
  2. 2.California NanoSystems Institute, UCLALos AngelesUSA
  3. 3.Jonsson Comprehensive Cancer Center, Broad Center of Regenerative Medicine and Stem Cell Research, and Molecular Biology Institute, UCLALos AngelesUSA
  4. 4.Veeco Instruments, Inc.TucsonUSA
  5. 5.Department of Chemistry and BiochemistryUCLALos AngelesUSA

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