Renin Cell Identity and Homeostasis
Renin-expressing cells are precursors for vascular smooth muscle cells within the kidney, which in turn are capable of dedifferentiating and synthesize renin in response to challenges to homeostasis. The mechanisms that determine the identity of renin cells are the subject of many studies. Using a cell model of renal arteriolar smooth muscle cells dually labeled with fluorescent markers we showed that in vitro the cells can reexpress renin upon cAMP stimulation. In addition, reexpression of renin through the cAMP pathway involves chromatin remodeling by acetylation of histone 4. Further experiments confirmed that histone acetyl transferases play an essential role in the maintenance of the renin cell identity.
KeywordsYellow Fluorescent Protein Cyan Fluorescent Protein cAMP Pathway cAMP Responsive Element Histone Acetyl Transferase
- 16.Sequeira Lopez ML, Pentz ES, Robert B, Abrahamson DR, Gomez RA. Embryonic origin and lineage of juxtaglomerular cells. Am J Physiol Renal Physiol. Aug 2001;281(2):F345–F356.Google Scholar
- 20.Taugner R, Hackenthal E. The Juxtaglomerular Apparatus: Structure and Function. Heidelberg: Springer Verlag; 1989.Google Scholar