Kainic Acid-Induced Cell Proliferation in the Striatum Is Not Estrogen Dependent
In the past decade it has become increasingly clear that the adult central nervous system has the ability to generate new neuronal cells. In particular, the subgranular layer (SGL) in the hippocampal dentate gyrus and the subventricular zone (SVZ) lining the lateral ventricles appear to be specially suited for this purpose. In these areas neurogenesis is observed due to the persistence of neuronal progenitors throughout life. The neuroprotective and neurogenic properties of estrogen have been demonstrated in vitro and in vivo. In this study we examined the role of estradiol benzoate (0, 25, and 100 μg per rat) in neuronal proliferation in the kainic acid (KA) rat model of Huntington’s disease. Cell proliferation and phenotype of newborn cells were assessed using antibodies against bromodeoxyuridine (BrdU), doublecortin, and glial fibrillary acidic protein in the adult female rat striatum, SVZ of the lateral ventricle, and corpus callosum, at different antero-posterior levels. The statistical analysis for the number of BrdU-positive cells in these cerebral regions showed that estradiol did not increase cellular proliferation beyond that induced by the KA lesion and that ovariectomy did not inhibit the proliferative response.
KeywordsOlfactory Bulb Neural Stem Cell Kainic Acid Adult Neurogenesis Rostral Migratory Stream
The authors wish to thank E.N. Hernández, Soledad Mendoza, and Ulises G. Pacheco for technical assistance. This work was supported by grants IN225305 from PAPIIT-DGAPA and 46161-M, 41477-Q from CONACyT.
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