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Blockade of GABA Transporter (GAT-1) Modulates the GABAergic Transmission in the Rat Globus Pallidus

  • Xiao-Tao Jin
  • Jean-Francois Paré
  • Yoland Smith
Conference paper
Part of the Advances in Behavioral Biology book series (ABBI, volume 58)

Abstract

Previous studies from our laboratory have demonstrated that application of SKF 89976A, a selective inhibitor of the GABA transporter GAT-1, reduces the activity of pallidal neurons in monkeys. However, the functional role of GAT-1 on GABAergic synaptic transmission in the globus pallidus (GP) is poorly understood. In the present study, we applied the whole-cell patch clamp recording technique to study the effects of blockade of GAT-1 on GABAA receptor-mediated inhibitory postsynaptic currents (IPSCs) recorded from rat GP slice preparations. Under maximal striatal stimulation (15–20 V) in parasagittal slices, SKF 89976A (10 μM) significantly prolonged the decay time, without significant effect on the amplitude, of IPSC. In contrast, SKF 89976A increased the amplitude, but did not prolong the decay time, of IPSCs under minimal striatal stimulation (2–5 V). We did not find any significant effect of SKF 89976A on IPSCs evoked locally from GP coronal slices. Furthermore, neither the amplitude nor the frequency of miniature IPSCs were changed following bath application of SKF 89976A. These results demonstrate that GABA reuptake through GAT-1 plays a major activity-dependent role in regulating GABAergic transmission at striatopallidal synapses in the GP.

Keywords

Globus Pallidus Bath Application GABAergic Transmission Gaba Transporter Inhibitory Postsynaptic Current 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a grant from NIH to YS (R01 NS 042937) and the Yerkes base grant (RR00165).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xiao-Tao Jin
    • 1
  • Jean-Francois Paré
    • 1
  • Yoland Smith
    • 1
  1. 1.Division of Neuroscience, Yerkes National Primate Research Center and Department of NeurologyEmory UniversityAtlantaUSA

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