Molecular Genetics at the T-Cell Receptor β Locus: Insights into the Regulation of V(D)J Recombination

  • Marie Bonnet
  • Pierre Ferrier
  • Salvatore Spicuglia
Part of the Advances in Experimental Medicine and Biology book series (volume 650)


The V(D)J recombination machinery assembles antigen receptor genes from germline V,D and J segments during lymphocyte development. In αβT cells, this leads to the production of the T-cell receptor (TCR) α and β chains. Notably, V(D)J recombination at the Tcrb locus is tightly controlled at various levels, including cell-type and stage specificities, intralocus ordering and allelic exclusion. Although many of these controls are partly mediated at the level of genomic accessibility to the V(D)J recombinase, recent studies have uncovered novel mechanisms that are also likely to contribute to the developmental regulation of Tcrb gene rearrangement events. In this chapter, we summarize our current knowledge and highlight unanswered questions regarding the regulation of V(D)J recombination at the Tcrb locus, placing emphasis on mouse transgenesis and gene-targeting approaches.


Gene Segment Chromatin Accessibility Recombination Signal Sequence Allelic Exclusion Plant Homeodomain Finger 
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Copyright information

© Landes Bioscience and Springer Science+Business Media 2009

Authors and Affiliations

  • Marie Bonnet
    • 1
  • Pierre Ferrier
    • 1
  • Salvatore Spicuglia
    • 1
  1. 1.Centre d’Immunologie de Marseille-LuminyUniversité d’Aix Marseille CNRS, UMR 6102 Inserm, U 631MarseilleFrance

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