Background Extraction in Electron Microscope Images of Artificial Membranes

  • A. Karathanou
  • J.-L. Buessler
  • H. Kihl
  • J.-P. Urban
Conference paper
Part of the IFIP International Federation for Information Processing book series (IFIPAICT, volume 296)


On-line analysis of Transmission Electron Microscope (TEM) images is a field with great interest that opens up new prospects regarding automatic acquisitions. Presently, our work is focused on the automatic identification of artificial membranes derived from 2D protein crystallization experiments. Objects recognition at medium magnification aims to control the microscope in order to acquire interesting membranes at high magnification. A multiresolution segmentation technique has been proposed for the image partition. This paper presents an analysis of this partition to extract the background. To achieve this goal in very noisy images, it is essential to suppress false contours as they split the background into multiple regions. Statistical properties of such regions are not always sufficient for their identification as background. The analysis of these regions contours was therefore considered. In the proposed solution, the elimination of false contours is based on the statistical examination of the perpendicular gradient component along the contour. After this improved segmentation, the background extraction can be easily effectuated since this resulting region appears bright and large.


Transmission Electron Microscope Image Electron Microscope Image Background Region Membrane Region Artificial Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© IFIP International Federation for Information Processing 2009

Authors and Affiliations

  • A. Karathanou
    • 1
  • J.-L. Buessler
    • 1
  • H. Kihl
    • 1
  • J.-P. Urban
    • 1
  1. 1.MIPS laboratoryUniversity of Haute Alsace, 4 rue des Frères LumièreMulhouse CedexFrance

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