• Dongwu Liu
  • Zhiwei Chen
  • Hongzhi Xu
  • Wenqi Liu
  • Lina Wang
Conference paper
Part of the IFIP Advances in Information and Communication Technology book series (IFIPAICT, volume 293)


Confocal microscopy, which is a major advance upon normal light microscopy, has been used in a number of scientific fields. By confocal microscopy techniques, cells and tissues can be visualized deeply, and three-dimensional images created. Compared with conventional microscopes, confocal microscope improves the resolution of images by eliminating out-of-focus light. Moreover, confocal microscope has a higher level of sensitivity due to highly sensitive light detectors and the ability to accumulate images captured over time. In present studies, a series of Weeping Forsythia pollen digital images (35 images in total) were acquired with confocal microscope, and the three-dimensional digital image of the pollen reconstructed with confocal microscope. Our results indicate that it's a very easy job to analysis threedimensional digital image of the pollen with confocal microscope and the probe Acridine orange (AO).


Acridine Orange Middle Jurassic Reactive Nitrogen Species Middle Triassic Acridine Orange 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Dongwu Liu
    • 1
  • Zhiwei Chen
    • 1
  • Hongzhi Xu
    • 1
  • Wenqi Liu
    • 2
  • Lina Wang
    • 1
  1. 1.Analysis and Testing CenterShandong University of TechnologyZiboP. R.China
  2. 2.School of Life SciencesShandong University of TechnologyZiboP. R.China

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