This chapter discusses challenges and practical approaches to animal fMRI with respect to anesthetic regimens, conscious animal studies, activation paradigms, optimal pulse sequence selection, and the rigor required for drug discovery applications. Examples on the use of animal fMRI to support drug discovery are presented. The examples illustrate (1) technical development and qualification of a quantitative assay for pain, (2) the use of pharmacological tools to support a link between a receptor antagonist elicited ASL-CBF change with the corresponding neuronal activity, and (3) interrogation of mechanism of action of an analgesic.
KeywordsCerebral Blood Flow Neuropathic Pain Cerebral Blood Volume Arterial Spin Label fMRI Signal
We acknowledge the contributions of Denise Welsh to the unpublished data presented here. We thank Jeffery Evelhoch and Richard Hargreaves for their valuable input.
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