Abstract
We isolated 4 lupane triterpenes as differentiation-inducing agents on B16 2F2 melanoma cells from Lactuca indica. In the study of structure-activity relationships, the differences of the lupane skeleton at C-3 played a key role in their melanogenic activities. SB203580, a selective inhibitor of p38 MAPK, completely blocked the melanogenesis of B16 2F2 cells induced by lupeol (1), a lupane triterpene. Western blot analysis revealed that 1 transiently activated p38 MAPK. Furthermore, 1 was found to induce dendritic formation, a morphological indicator of B16 2F2 cell differentiation. 1 attenuated actin fiber assembly in B16 2F2 cells, and this attenuation caused dendrite outgrowths of the cells. SB203580 blocked the induction of tyrosinase by 1, but did not block the rearrangement of actin cytoskeleton in B16 2F2 cells stimulated with 1.
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Acknowledgments
This research was supported in part by a grant from the Department of Academic and International Affairs, Akita Prefecture Government.
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Hata, K. et al. (2008). Differentiation-Inducing Activities by Lupane Triterpenes from Lactuca indica on a Mouse Melanoma Cell Line. In: Shirahata, S., Ikura, K., Nagao, M., Ichikawa, A., Teruya, K. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9646-4_43
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DOI: https://doi.org/10.1007/978-1-4020-9646-4_43
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