Advertisement

Nuclear Structures Regulate Liver-Specific Expression of the Tryptophan Oxygenase Gene

  • Hidenori Kaneoka
  • Katsuhide Miyake
  • Shinji Iijima
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 15)

Abstract

The gene for tryptophan oxygenase (TO) is expressed in a tissue- and development-specific manner and is regulated by glucocorticoid hormone in rat liver. Transcription of the gene is induced 10-fold by glucocorticoids. The TO promoter has two glucocorticoid-responsive elements (GRE), located 450 bp and 1.2 kb upstream from the cap site, and two TATA boxes. Combination of nuclear fractionation and quantitative PCR analysis showed that a putative matrix attachment region, which resides 8.5 kb upstream of the promoter, bound to nuclear matrix in the hormone-treated adult hepatocytes. However, this DNA region was partly released from the nuclear matrix by removing the hormone. These results indicated that binding of MAR to nuclear matrix was also important for regulation of the TO gene expression.

Keywords

Tryptophan oxygenase glucocorticoid receptor hepatocyte 

References

  1. 1.
    Iarovaia, O. V., Akopov, S. B., Nikolaev, L. G., Sverdlov, E. D., and Razin, S. V. (2005) Induction of transcription within chromosomal DNA loops flanked by MAR elements causes an association of loop DNA with the nuclear matrix. Nucleic Acids Res. 13, 4157–4163.CrossRefGoogle Scholar
  2. 2.
    Heng, H. H., Goetze, S., Ye, C. J., Liu, G., Stevens, J. B., Bremer, S. W., Wykes, S. M., Bode, J., and Krawetz, S. A. (2004) Chromatin loops are selectively anchored using scaffold/matrix-attachment regions. J. Cell. Sci. 117, 999–1008.CrossRefPubMedGoogle Scholar
  3. 3.
    Cockerrill, P. N. and Garrard, W. T. (1986) Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44, 273–282.CrossRefGoogle Scholar
  4. 4.
    Luderus, M. E., de Graaf, A., Mattia, E., den Blaauwen, J. L., Grande, M. A., de Jong, L., and van Driel, R. (1992) Binding of matrix attachment regions to lamin B1. Cell 70, 949–959.CrossRefPubMedGoogle Scholar
  5. 5.
    Romig, H., Fackelmayer, F. O., Renz, A., Ramsperger, U., and Richter, A. (1992) Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements. EMBO J. 11, 3431–3440.PubMedGoogle Scholar
  6. 6.
    Hebbar, P. B. and Archer, T. K. (2003) Chromatin remodeling by nuclear receptors. Chromosoma 111, 495–504.CrossRefPubMedGoogle Scholar
  7. 7.
    Eggert, M., Michel, J., Schneider, S., Bornfleth, H., Baniahmad, A., Fackelmayer, F. O., Schmidt, S., and Renkawitz, R. (1997) The glucocorticoid receptor is associated with the RNA-binding nuclear matrix protein hnRNP U. J. Biol. Chem. 272, 28471–28478.CrossRefPubMedGoogle Scholar
  8. 8.
    Danesch, U., Gloss, B., Schmid, W., Schütz, G., Schüle, R., and Renkawitz, R. (1987) Glucocorticoid induction of the rat tryptophan oxygenase gene is mediated by two widely separated glucocorticoid-responsive elements. EMBO J. 6, 625–630.PubMedGoogle Scholar
  9. 9.
    Hamamoto, R., Yamada, K., Kamihira, M., and Iijima, S. (1998) Differentiation and proliferation of primary rat hepatocytes cultured as spheroids. J. Biochem. 124, 972–979PubMedGoogle Scholar
  10. 10.
    Dohda, T., Nakamura, Y., Kamihira, M., and Iijima, S. (2004) Functional role of RhoA in growth regulation of primary hepatocytes. J. Biochem. 135, 631–637.CrossRefPubMedGoogle Scholar
  11. 11.
    Mirkovitch, J., Mirault, M. E., and Laemmli, U. K. (1994) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39, 223–232.CrossRefGoogle Scholar
  12. 12.
    Kaltschmidt, C., Muller, M., Brem, G., and Renkawitz, R. (1994) DNase I hypersensitive sites far upstream of the rat tryptophan oxygenase gene direct developmentally regulated transcription in livers of transgenic mice. Mech. Dev. 45, 203–210.CrossRefPubMedGoogle Scholar
  13. 13.
    Cremer, T. and Cremer, C. (2001) Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292–301.CrossRefPubMedGoogle Scholar
  14. 14.
    Zaidi, S. K., Young, D. W., Choi, J. Y., Pratap, J., Javed, A., Montecino, M., Stein, J. L., van Wijnen, A. J., Lian, J. B., and Stein, G. S. (2005) The dynamic organization of gene-regulatory machinery in nuclear microenvironments. EMBO Rep. 6, 128–133.CrossRefPubMedGoogle Scholar
  15. 15.
    Osborne, C. S., Chakalova, L., Brown, K. E., Carter, D., Horton, A., Debrand, E., Goyenechea, B., Mitchell, J. A., Lopes, S., Reik, W., and Fraser, P. (2004) Active genes dynamically colocalize to shared sites of ongoing transcription. Nat. Genet. 36, 1065–1071.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Hidenori Kaneoka
    • 1
  • Katsuhide Miyake
    • 1
  • Shinji Iijima
    • 1
  1. 1.Department of Biotechnology, Graduate School of EngineeringNagoya UniversityFuro-cho, Chikusa-kuJapan

Personalised recommendations