Abstract
Organotin compounds, particularly tributyltin chloride (TBT) and dibutyltin dichloride (DBT), are widely distributed toxicants. They inhibit cell proliferation and are known to cause neurotoxicity and genotoxicity in animals and humans. DBT is used as a catalyst in biodegradable polymers. We evaluated the effects of TBT and DBT on chondrogenesis of human articular chondrocytes (HAC) under a micromass culture system. In 4 weeks of culture, the lowest dose of TBT caused an increase in cell proliferation and differentiation. When the dose was increased, its effect on cultured chondrocytes was inhibitory compared with the control cultures. DBT produced little change in cell proliferation but it significantly inhibited cell differentiation compared with the control cultures. The expression of cartilage-specific genes, namely collagen type II and aggrecan was inhibited in TBT- and DBT-treated cultured chondrocytes. TBT was significantly toxic to HAC at 0.16 ppb and DBT at 0.75 ppb.
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We are grateful for the support of grants for Research on Advanced Medical Technology from the Ministry of Health, Labour and Welfare and the Japan Health Sciences Foundation.
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© 2008 Springer Science+Business Media B.V.
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Banu, N., Tsuchiya, T., Sawada, R. (2008). Effects of Tin Compounds on Human Chondrogenic Activity In Vitro. In: Shirahata, S., Ikura, K., Nagao, M., Ichikawa, A., Teruya, K. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9646-4_29
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DOI: https://doi.org/10.1007/978-1-4020-9646-4_29
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