Abstract
Plant cell walls are a pivotal battleground between microbial pathogens and their plant hosts. Microbial pathogens secrete an array of cell-wall-degrading enzymes to depolymerize the noncellulosic polysaccharides of primary cell walls, which are essential for the vitality of pathogens, either saprophytically, pathogenetically or both. The predicted protein encoded by MGG_01403.5 from Magnaporthe grisea has a high degree of sequence identity with the ferulic acid esterase A (so to be designated as MgFaeA) from Penicillium funiculosum. Tested by the SignalP, TMHMM and Protcomp programs, the 284-amino acid hypothetical protein contained a cleavable signal peptide at the N terminus, lacked transmembrane structure, and appeared located in the extracellular matrix. In the work presented here, MgFaeA, with a His6 tag at its C-terminus, was introduced into M. grisea for overexpression. The MgFaeA-His6 fusion protein was purified from the culture filtrate, which confirmed the secretion property of MgFaeA in the fungus. To further investigate the role of MgFaeA on rice infection, RT-PCR was used to analyze the gene expression during different stages of infection. The results indicated that MgFaeA transcripts were detectable as early as 72 hpi, and reached to peak at 7 dpi. It suggested that the MgFaeA gene might be involved in the fungal pathogenesis.
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References
Apel-Birkhold, P.C., & Walton, J.D. (1996). Cloning, disruption,and expression of two endo-β-1,4-xylanase genes, XYL2 and XYL3, from Cochliobolus carbonum. Appl Environ Microbiol, 62, 4129–4135.
Bailey, B.A., Dean, J.F.D., & Anderson, J.D. (1990). An ethylene biosynthesis-inducing endoxylanase elicits electrolyte leakage and necrosis in Nicotiana tabacum cv. xanthiliaves. Plant Physiol, 94, 1849–1854.
Cervone, F., De Lorenzo, G., Degra, L., & Salvi, G. (1987). Elicitation of necrosis in Vigna unguiculata Walp. By homogeneous Aspergillus niger endo-polygalacturonase and by αD-galacturonate oligomers. Plant Physiol, 85, 626–630.
Chen, J.S., Zheng, S.Q., Zheng, W., Zhou, J., Lu, G.D., & Wang, Z.H. (2006). Prediction for secreted proteins from Magnaporthe grisea Genome. Sci Agr Sinica, 39, 2474–2482.
Darvill, A., Augur, C., Bergmann, C., Carlson, R.W., Cheong, J.-J., Eberhard, S., Hahn, M.G., Lo’, V.-M., Marfa‘, V., Meyer, B., Mohnen, D., O’Neill, M.A., Spiro, M.D., Van Halbeek, H., York, W.S., & Albersheim, P. (1992). Oligosaccharins-oligosaccharides that regulate growth, development and defence responses in plants. Glycobiol, 2, 181–198.
de Vries, R.P., Michelsen, B., Poulsen, C.H., Kroon, P.A., van den Heuvel, R.H.H., Faulds, C.B., Williamson, G., van den Hombergh, J.P.T.W., & Visser, J., (1997). The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in the degradation of complex cell wall polysaccharides. Appl Environ Microbiol, 63,4638–4644.
de Vries, R.P., & Visser, J., (2001). Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microb Mol Biol Rev, 65, 497–522.
Dean, J.F.D., & Anderson, J.D. (1991). Ethylene biosynthesis-inducing xylanase. Plant Physiol, 95, 316–323.
Dean, R., et al. (2005). The genome sequence of the rice blast fungus Magnaporthe grisea. Nature, 434, 980–986.
Faulds, C.B., Molina, R., Gonzalez, R., Husband, F., Juge, N., Sanz-Aparicio, J., & Hermoso, J.A. (2005). Probing the determinants of substrate specificity of a feruloyl esterase, AnFaeA, from Aspergillus niger. Eur J Biochem, 272, 4362–4371.
Gilkes, N.R., Henrissat, B., Kilburn, D.G., Miller, R.C., & Warren, R.A.J. (1991). Domains in microbial beta-1,4-glycanases-sequence conservation, function and enzyme families. Microbiol Rev, 55, 303–315.
Guo, W.J., Gonzalez-Candelas, L., & Kolattukudy, P.E. (1996). Identification of a novel pelD gene expressed uniquely in planta by Fusarium solani f.sp. pisi (Nectria haematococca, mating type VI) and characterization of its protein product as an endo-pectate lyase. Arch. Biochem. Biophys, 332, 305–312.
Ham, K.-S., Wu, S.-C., Darvill, A. G., & Albersheim, P. (1997). Fungal pathogens secrete an inhibitor protein that distinguishes isoforms of plant pathogenesis-related endo-β-1,3-glucanases. Plant J, 11, 169–179.
Ishii, T. (1997). Structure and function of feruloylated polysaccharides. Plant Sci, 127, 111–127.
Kroon, P.A., Williamson, G., Fish, N.M., Archer, D.B., & Belshaw, N.J. (2000). A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module. Eur J Biochem, 267,6740–6752.
Liu, P.Z., Ou, S.Y., Bao, H.Y. (2006). Research and application of ferulic acid esterase. Food Res. Dev, 127, 169–171.
Lu, G.D., Filippi, C., Li, D., et al. (2004). Identification and characterization of secreted proteins from Magnaporthe grisea. In: Shinji Kawasaki (Eds) Rice blast: interaction with rice and control, (pp. 47–55). Sheffied: Kluwer Academic Publishers, Dordrecht, The Netherlands.
Margolles-Clark, E., Tenkanen, M., SoÈderlund, H., & PenttilaÈ, M. (1996). Acetyl xylan esterase from Trichoderma reesei contains an active site serine residue and a cellulose-binding domain. Eur J Biochem, 237, 553–560.
Reid, J. G. (2000). Cementing the wall: cell wall polysaccharide synthesising enzymes. Curr Opin Plant Biol, 3, 512–516.
Rumbold, K., Biely, P., Mastihubova’, M., Gudelj, M., Gübitz, G., Robra, K.-H., & Prior, B. A. (2003). Purification and properties of a feruloyl esterase involved in lignocellulose degradation by Aureobasidium pullulans. Appl. Environ. Microbiol, 69, 5622–5626.
Rumbold, K., Okatch, H. Torto, N., Siika-Aho, M., Gubitz, G.M., Robra, K.-H., & Prior, B. A. (2002). Monitoring on-line desalted lignocellulosic hydrolysates by microdialysis sampling micro-high performance anion exchange chromatography with integrated pulsed electrochemical detection/mass spectrometry. Biotechnol. Bioeng, 78, 822–828.
Talbot, N.J. (2003). On the trail of a cereal killer: exploring the biology of Magnaporthe grisea. Annu Rev Microbiol, 57, 177–202.
Ten Have, A., Mulder, W. Visser, J., & van Kan, J.A. (1998). The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol. Plant-Microbe Interact, 11,1009–1016.
Tonukari, N.J., Scott-Craig, J.S., & Walton, J.D. (2000). The Cochliobolus carbonum SNF1 gene is required for cell wall-degrading enzyme expression and virulence on maize. Plant Cell, 12, 237–247.
Walton, J.D. (1994). Deconstructing the cell wall. Plant Physiol, 104, 1113–1118.
Wu, S.-C., Ham, K.-S., Darvill, A.G., & Albersheim, P. (1997). Deletion of two endo-β-1,4-xylanase genes reveals additional isozymes secreted by the rice blast fungus. Mol. Plant-Microbe Interact, 10, 700–708.
Wu, S.-C., Halley, J. E., Luttig, C., & Fernekes, L. M., et al. (2006). Identification of an endo-β-1,4-D-xylanase from Magnaporthe grisea by gene knockout analysis, purification, and heterologous expression. Appl Environ Microbiol, 72, 986–993.
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Zheng, Xz. et al. (2009). Secretion Property and Gene Expression Pattern of a Putative Feruloyl Esterase in Magnaporthe grisea . In: Wang, GL., Valent, B. (eds) Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9500-9_5
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DOI: https://doi.org/10.1007/978-1-4020-9500-9_5
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