Non-Conventional Generation and Transformation of Response
Most of our discussions in previous chapters were concentrated on sensors that can be excited by light and emit an informative light signal about the target binding. Meanwhile, this is not the only possibility for obtaining reporting information. Dyes, or luminophores in general, can also be excited in a chemical reaction (chemiluminescence), in a biochemical transformation (bioluminescence) and in a reaction at an electrode (electroluminescence and more specifically, electrochemi-luminescence). The reporting can be provided by deactivation of the excited state, not only in the form of emission but also in the form of electron transfer to the conducting surface. This allows producing an electrical signal directly, avoiding emission and detection of light. Moreover, the emission of a miniaturized semiconductor or polymeric light source can be directly coupled with the sensing event. Such coupling can be provided with the response of a miniaturized detector.
Excitation via the evanescent field effect is a powerful tool to introduce spatial resolution into the sensor system; it can be combined with different sensing technologies. If introduced into heterogeneous assays, it allows a direct response to the target binding.
KeywordsPeroxide Manifold Estrogen Superoxide Adenosine
Unable to display preview. Download preview PDF.
- Tokuyama H, Nakamura M (2005) Acceleration of reaction by microwave irradiation. Journal of Synthetic Organic Chemistry Japan 63:523–538Google Scholar
- Xu Y, Cai H, Liu QJ, Qin LF, Wang LJ, Wang P (2006) A novel structure of LAPS array for cell-based biosensor. Rare Metal Materials and Engineering 35:51–54Google Scholar
- Yashchuk VM, Gusak VV, Drnytruk IM, Prokopets VM, Kudrya VY, Losytskyy MY, Tokar VP, Gumenyuk YO, Yarmoluk SM, Kovalska VB, Balanda AO, Kryvorotenko DV (2007) Two-photon excited luminescent styryl dyes as probes for the DNA detection and imaging. Photostability and phototoxic influence on DNA. Molecular Crystals and Liquid Crystals 467:325–338CrossRefGoogle Scholar