Abstract
This chapter reviews the proteomics research performed to elucidate the molecular processes associated with embryo initiation and development in Brassica napus (rapeseed) microspore-derived embryo cultures. Classical biochemical methods such as combined [35S]-methionine labelling and 2-D gel electrophoresis (2-DE) have been used for the large-scale study of proteins differentially expressed during the inductive phase of embryogenesis. Immuno-cytological experiments have been carried out to study the (sub)cellular localization of putative marker proteins for embryo induction. Changes in post-translational modification of proteins (phosphorylation) have been detected during the induction phase, as have changes in the profile of proteins en peptides secreted by developing embryos. We also discuss recent developments in quantitative proteomics technologies such as fluorescence labelling of protein samples and stable isotope labelling by amino acids in cell cultures that enable more detailed expression analyses and the identification of low abundant proteins in complex mixtures.
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Cordewener, J., van der Wal, F., Joosen, R., Boutilier, K., America, T. (2009). Proteomics in Rapeseed Microspore Embryogenesis. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_10
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DOI: https://doi.org/10.1007/978-1-4020-8854-4_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8853-7
Online ISBN: 978-1-4020-8854-4
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