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De novo Designed Bacteriochlorophyll-Binding Helix-Bundle Proteins

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The Purple Phototrophic Bacteria

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 28))

Summary

The construction of small synthetic proteins that bind only one or a small number of (bacterio)chlorophylls ((B)Chls) is a powerful approach to understand the concepts and guidelines of protein-(B)Chl interactions and assembly; the systems can be extended, in a stepwise fashion, by adding other cofactors or by oligomerization. We review the unique aspects of the de novo design and construction of (B)Chl-binding proteins, and describe recent progress and challenges in designing new BChl-protein platforms for delineating general rules and guidelines of (B)Chl-protein assembly, structure, and function. The relevant aspects of chlorophyll (Chl) and BChl chemical structures are outlined, as well as the modes of interactions with natural proteins. Two distinct strategies are then described for designing de novo water-soluble (B)Chl-binding proteins. The first strategy is based on the covalent assembly of modular four-helix bundle proteins, the second follows the original non-covalent heme-binding protein maquette design, which relies on self-assembly of amphiphilic helices that is primarily driven by the hydrophobic effect. Finally, we demonstrate the extension of the latter to designing transmembrane-like versions of (B)Chl-protein maquettes.

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Abbreviations

AP:

amphiphilic

BChl:

bacteriochlorophyll

BChlide:

bacteriochlorophyllide

BPhe:

bacteriopheophytin

BPheide:

bacteriopheophorbide

CD:

circular dichroism

Chi:

chlorophyll

Chlide:

chlorophyllide

HP:

hydrophilic

LP:

lipophilic

[M]-(B)Chlide:

(B)Chlide in which the central metal has been replaced by metal ‘M’

MOP:

modular organized protein

NIR:

near infrared

Phe:

pheophytin

RC:

reaction center

UV:

ultraviolet

Vis:

visible

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Authors and Affiliations

  1. Institut für Biologie - Biochemie der Pflanzen, Universität, Schänzlestr. 1, D-79104, Freiburg, Germany

    Wolfgang Haehnel

  2. Plant Sciences Department, Weizmann Institute of Science, Rehovot, 76100, Israel

    Dror Noy

  3. Department Biologie I – Botanik, Menzinger Str. 67, D-80638, München, Germany

    Hugo Scheer

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  1. Wolfgang Haehnel
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  2. Dror Noy
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  3. Hugo Scheer
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Correspondence to Hugo Scheer .

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Editors and Affiliations

  1. University of Sheffield, UK

    C. Neil Hunter

  2. University of Pennsylvania, USA

    Fevzi Daldal

  3. Argonne National Laboratory, USA

    Marion C. Thurnauer

  4. University of British Columbia, Canada

    J. Thomas Beatty

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Haehnel, W., Noy, D., Scheer, H. (2009). De novo Designed Bacteriochlorophyll-Binding Helix-Bundle Proteins. In: Hunter, C.N., Daldal, F., Thurnauer, M.C., Beatty, J.T. (eds) The Purple Phototrophic Bacteria. Advances in Photosynthesis and Respiration, vol 28. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8815-5_45

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