Molecular approaches for characterization and use of natural disease resistance in wheat

  • Navreet Kaur
  • Kenneth Street
  • Michael Mackay
  • Nabila Yahiaoui
  • Beat Keller


Wheat production is threatened by a constantly changing population of pathogen species and races. Given the rapid ability of many pathogens to overcome genetic resistance, the identification and practical implementation of new sources of resistance is essential. Landraces and wild relatives of wheat have played an important role as genetic resources for the improvement of disease resistance. The use of molecular approaches, particularly molecular markers, has allowed better characterization of the genetic diversity in wheat germplasm. In addition, the molecular cloning of major resistance (R) genes has recently been achieved in the large, polyploid wheat genome. For the first time this allows the study and analysis of the genetic variability of wheat R loci at the molecular level and therefore, to screen for allelic variation at such loci in the gene pool. Thus, strategies such as allele mining and ecotilling are now possible for characterization of wheat disease resistance. Here, we discuss the approaches, resources and potential tools to characterize and utilize the naturally occurring resistance diversity in wheat. We also report a first step in allele mining, where we characterize the occurrence of known resistance alleles at the wheat Pm3 powdery mildew resistance locus in a set of 1,320 landraces assembled on the basis of eco-geographical criteria. From known Pm3 R alleles, only Pm3b was frequently identified (3% of the tested accessions). In the same set of landraces, we found a high frequency of a Pm3 haplotype carrying a susceptible allele of Pm3. This analysis allowed the identification of a set of resistant lines where new potentially functional alleles would be present. Newly identified resistance alleles will enrich the genetic basis of resistance in breeding programmes and contribute to wheat improvement.


Allele mining Genetic diversity Pm3 Wheat powdery mildew 


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  1. Adhikari, T. B., Anderson, J. M., & Goodwin, S. B. (2003). Identification and molecular mapping of a gene in wheat conferring resistance to Mycosphaerella graminicola. Phytopathology, 93, 1158–1164.PubMedCrossRefGoogle Scholar
  2. Allard, R. W., & Shands, R. G. (1954). Inheritance of resistance to stem rust and powdery mildew in cytologically stable spring wheat derived from Triticum timopheevi. Phytopathology, 44, 266–274.Google Scholar
  3. Bariana, H. S., Hayden, M. J., Ahmed, N. U., Bell, J. A., Sharp, P. J., & McIntosh, R. A. (2001). Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Australian Journal of Agricultural Research, 52, 1247–1255.CrossRefGoogle Scholar
  4. Baum, M., Lagudah, E. S., & Appels, R. (1992). Wide crosses in cereals. Annual Review of Plant Physiology and Plant Molecular Biology, 43, 117–143.CrossRefGoogle Scholar
  5. Bossolini, E., Krattinger, S. G., & Keller, B. (2006). Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theoretical and Applied Genetics, 113, 1049–1062.PubMedCrossRefGoogle Scholar
  6. Chagué, V., Fahima, T., Dahan, A., Sun, G. L., Korol, A. B., Ronin, Y. I., et al. (1999). Isolation of microsatellite and RAPD markers flanking Yr15 gene of wheat using NILs and bulked segregant analysis. Genome, 42, 1050–1056.PubMedCrossRefGoogle Scholar
  7. Chen, Y. P., Wang, H. Z., Cao, A. Z., Wang, C. M., & Chen, P. D. (2006). Cloning of a resistance gene analog from wheat and development of a codominant PCR marker for Pm21. Journal of Integrative Plant Biology, 48, 715–721.CrossRefGoogle Scholar
  8. Cloutier, S., McCallum, B. D., Loutre, C., Banks, T. W., Wicker, T., Feuillet, C., et al. (2007). Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Molecular Biology, 65, 93–106.PubMedCrossRefGoogle Scholar
  9. Comai, L., Young, K., Till, B. J., Reynolds, S. H., Greene, E. A., Codomo, C. A., et al. (2004). Efficient discovery of DNA polymorphisms in natural populations by Ecotilling. The Plant Journal, 37, 778–786.PubMedCrossRefGoogle Scholar
  10. De Bustos, A., & Jouve, N. (2003). Characterisation and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale. Theoretical and Applied Genetics, 107, 74–83.PubMedGoogle Scholar
  11. Feuillet, C., Travella, S., Stein, N., Albar, L., Nublat, A., & Keller, B. (2003). Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome. Proceedings of the National Academy of Sciences of the United States of America, 100, 15253–15258.PubMedCrossRefGoogle Scholar
  12. Forsström, P., & Merker, A. (2001). Sources of wheat powdery mildew resistance from wheat-rye and wheat-Leymus hybrids. Hereditas, 134, 115–119.PubMedCrossRefGoogle Scholar
  13. Griffiths, S., Sharp, R., Foote, T. N., Bertin, I., Wanous, M., Reader, S., et al. (2006). Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat. Nature, 439, 749–752.PubMedCrossRefGoogle Scholar
  14. Gupta, P. K., Varshney, R. K., Sharma, P. C., & Ramesh, B. (1999). Molecular markers and their applications in wheat breeding. Plant Breeding, 118, 369–390.CrossRefGoogle Scholar
  15. Helguera, M., Khan, I. A., & Dubcovsky, J. (2000). Development of PCR markers for the wheat leaf rust resistance gene Lr47. Theoretical and Applied Genetics, 100, 1137–1143.CrossRefGoogle Scholar
  16. Huang, L., Brooks, S. A., Li, W., Fellers, J. P., Trick, H. N., & Gill, B. S. (2003). Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics, 164, 655–664.PubMedGoogle Scholar
  17. Huang, L., & Gill, B. S. (2001). An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat. Theoretical and Applied Genetics, 103, 1007–1013.CrossRefGoogle Scholar
  18. Keller, B., Feuillet, C., & Yahiaoui, N. (2005). Map-based isolation of disease resistance genes from bread wheat: cloning in a supersize genome. Genetical Research, 85, 93–100.PubMedCrossRefGoogle Scholar
  19. Kota, R., Spielmeyer, W., McIntosh, R. A., & Lagudah, E. S. (2006). Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 112, 492–499.PubMedCrossRefGoogle Scholar
  20. Lagudah, E. S., McFadden, H., Singh, R. P., Huerta-Espino, J., Bariana, H. S., & Spielmeyer, W. (2006). Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics, 114, 21–30.PubMedCrossRefGoogle Scholar
  21. Latha, R., Rubia, L., Bennett, J., & Swaminathan, M. S. (2004). Allele mining for stress tolerance genes in Oryza species and related germplasm. Molecular Biotechnology, 27, 101–108.PubMedCrossRefGoogle Scholar
  22. Liu, S., & Anderson, J. A. (2003). Targeted molecular mapping of a major wheat QTL for Fusarium head blight resistance using wheat ESTs and synteny with rice. Genome, 46, 817–823.PubMedCrossRefGoogle Scholar
  23. Liu, Z., Sun, Q., Ni, Z., Nevo, E., & Yang, T. (2002). Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica, 123, 21–29.CrossRefGoogle Scholar
  24. Malysheva, L., Ganal, M. W., & Röder, M. S. (2004). Evaluation of cultivated barley (Hordeum vulgare) germplasm for the presence of thermostable alleles of β-amylase. Plant Breeding, 123, 128–131.CrossRefGoogle Scholar
  25. Mardi, M., Buerstmayr, H., Ghareyazie, B., Lemmens, M., Mohammadi, S. A., Nolz, R., et al. (2005). QTL analysis of resistance to Fusarium head blight in wheat using a ‘Wangshuibai’-derived population. Plant Breeding, 124, 329–333.CrossRefGoogle Scholar
  26. Miranda, L. M., Murphy, J. P., Marshall, D., & Leath, S. (2006). Pm34: A new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 113, 1497–1504.PubMedCrossRefGoogle Scholar
  27. Prasad, M., Varshney, R. K., Roy, J. K., Balyan, H. S., & Gupta, P. K. (2000). The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetics, 100, 584–592.Google Scholar
  28. Rong, J. K., Millet, E., Manisterski, J., & Feldman, M. (2000). A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica, 115, 121–126.CrossRefGoogle Scholar
  29. Rosewarne, G. M., Singh, R. P., Huerta-Espino, J., William, H. M., Bouchet, S., Cloutier, S., et al. (2006). Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theoretical and Applied Genetics, 112, 500–508.PubMedCrossRefGoogle Scholar
  30. Salvi, S., & Tuberosa, R. (2005). To clone or not to clone plant QTLs: Present and future challenges. Trends in Plant Science, 10, 297–304.PubMedCrossRefGoogle Scholar
  31. Scofield, S. R., Huang, L., Brandt, A. S., & Gill, B. S. (2005). Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiology, 138, 2165–2173.PubMedCrossRefGoogle Scholar
  32. Spielmeyer, W., McIntosh, R. A., Kolmer, J., & Lagudah, E. S. (2005). Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust co-segregate at a locus on the short arm of chromosome 7D on wheat. Theoretical and Applied Genetics, 111, 731–735.PubMedCrossRefGoogle Scholar
  33. Srichumpa, P., Brunner, S., Keller, B., & Yahiaoui, N. (2005). Allelic series of four powdery mildew resistance genes at the Pm3 locus in hexaploid bread wheat. Plant Physiology, 139, 885–895.PubMedCrossRefGoogle Scholar
  34. Stein, N., Feuillet, C., Wicker, T., Schlagenhauf, E., & Keller, B. (2000). Subgenome chromosome walking in wheat: A 450-kb physical contig in Triticum monococcum L. spans the Lr10 resistance locus in hexaploid wheat (Triticum aestivum L.). Proceedings of the National Academy of Sciences of the United States of America, 97, 13436–13441.Google Scholar
  35. Tommasini, L., Yahiaoui, N., Srichumpa, P., & Keller, B. (2006). Development of functional markers specific for seven Pm3 resistance alleles and their validation in the bread wheat gene pool. Theoretical and Applied Genetics, 114, 165–175.PubMedCrossRefGoogle Scholar
  36. Tyryshkin, L. G., Gul’tyaeva, E. I., Alpat’eva, N. V., & Kramer, I. (2006). Identification of effective leaf-rust resistance genes in wheat (Triticum aestivum) using STS markers. Russian Journal of Genetics, 42, 662–666.CrossRefGoogle Scholar
  37. William, M., Singh, R. P., Huerta-Espino, J., Islas, S. O., & Hoisington, D. (2003). Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology, 93, 153–159.PubMedCrossRefGoogle Scholar
  38. Xie, C., Sun, Q., Ni, Z., Yang, T., Nevo, E., & Fahima, T. (2004). Identification of resistance gene analogue markers closely linked to wheat powdery mildew resistance gene Pm31. Plant Breeding, 124, 198–200.CrossRefGoogle Scholar
  39. Yahiaoui, N., Brunner, S., & Keller, B. (2006). Rapid generation of new powdery mildew resistance genes after wheat domestication. The Plant Journal, 47, 85–98.PubMedCrossRefGoogle Scholar
  40. Yahiaoui, N., Srichumpa, P., Dudler, R., & Keller, B. (2004). Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat. The Plant Journal, 37, 528–538.PubMedCrossRefGoogle Scholar
  41. Yan, G. P., Chen, X. M., Line, R. F., & Wellings, C. R. (2003). Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theoretical and Applied Genetics, 106, 636–643.PubMedGoogle Scholar
  42. Zhang, P., Dreisigacker, S., Melchinger, A. E., Reif, J. C., Kazi, A. M., VanGinkel, M., et al. (2005). Quantifying novel sequence variation and selective advantage in synthetic hexaploid wheats and their backcross-derived lines using SSR markers. Molecular Breeding, 15, 1–10.CrossRefGoogle Scholar

Copyright information

© KNPV 2007

Authors and Affiliations

  • Navreet Kaur
    • 1
  • Kenneth Street
    • 2
  • Michael Mackay
    • 3
  • Nabila Yahiaoui
    • 1
    • 4
  • Beat Keller
    • 1
  1. 1.Institute of Plant BiologyUniversity of ZurichZürichSwitzerland
  2. 2.ICARDAAleppoSyria
  3. 3.Australian Winter Cereals CollectionCalalaAustralia
  4. 4.UMR Biologie et Génétique des Interactions Plante-Parasite CIRAD TA A-54/K Campus International de BaillarguetMontpellier cedex 15France

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