Sensors with Biorecognition Elements Entrapped into Silica Based Polymers
The paper presents characteristics of biosensors with biorecognition elements entrapped in silica based polymers developed and tested in the Institute of Chemical Process Fundamentals ASCR, Prague (ICPF). Prepolymerized alkoxysilanes were used for preparing whole cell biosensors of phenol, naphthalene or salicylic acid intermediate, and polychlorinated biphenyls (PCB). Modifications of prepolymer composition and immobilization procedure could increase viability of entrapped cells but can also influence sensors properties. UV curable polymers with silica skeleton, ORMOCER®s, have been matrices of a sensitive element of optical sensor for the in-situ continuous monitoring of glucose in bioreactors. This enzymatic sensor, developed within the project MATINOES, displayed 3 week stability during fermentation in a laboratory bioreactor.
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