Abstract
Development of needle discoloration and histological symptoms induced by Bursaphelenchus vallesianus were investigated in 3-year-old Pinus sylvestris plants. Seedlings were inoculated with B. vallesianus isolated from declining P. sylvestris in Valais, Switzerland. Plants were grown in the greenhouse at 28°C mean temperature and a low watering regime. The histological symptoms in the inoculated plants appeared as necrosis of parenchyma cells in the cortex, rays and cambium, about two weeks after inoculation. Tissue necrosis expanded to the xylem during the next days followed by the formation of cavitations. The external symptoms appeared as browning of needles on the stem and branches below the inoculation site, followed by wilting of the whole plant. Needle discoloration was delayed by few days to the internal symptoms. Our study with B. vallesianus demonstrated that external and internal symptoms followed a pattern similar to that caused by B. xylophilus and supported the hypothesis that the mechanism causing pine wilting is comparable among the pathogenic Bursaphelenchus species.
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References
Bakke, A., Anderson, R. and Kvamme, T. (1991). Pathogenicity of the nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus to Pinus sylvestris seedlings: A greenhouse test. Scandinavian Journal of Forest Research 6: 407–412.
Bolla, R. and Wood, R. (2004). Pine wood nematode: pathogenic or political? In The pine wood nematode, Bursaphelenchus xylophilus. Nematology Monographs and Perspectives, vol. 1 (eds. M. Mota and P. Vieira), pp. 31–54. E. J. Brill, Leiden, The Netherlands.
Braasch, H., Caroppo, S., Ambrogioni, L., Michalopoulos, H., Skarmoutsos, G. and Tomiczek, Ch. (1999). Pathogenicity of various Bursaphelenchus species to pines and implications to European forests. In Sustainability of Pine Forests in Relation to Pine Wilt and Decline. Proceedings of an International Symposium, Tokyo, Japan, 26–30 October 1998 (eds. K. Futai, K. Togashi and T. Ikeda), pp. 14–22. Shokado Shote, Kyoto, Japan.
Braasch, H., Schönfeld, U., Polomski, J. and Burgermeister, W. (2004). Bursaphelenchus vallesianus sp. n. – a new species of the Bursaphelenchus sexdentati group (Nematoda: Parasitaphelenchidae). Nematologia Mediterranea 32: 71–79.
Fukuda, K., Hogetsu, T. and Suzuki, K. (1994). Ethylene production during symptom development of pine-wilt disease caused by Bursaphelenchus xylopilus. European Journal of Forest Pathology 24: 193–202.
Ichihara, Y., Fukuda, K. and Suzuki, K. (2000). Early symptom development and histological changes associated with migration of Bursaphelenchus xylophilus in seedling tissues of Pinus thunbergii. Plant Disease 84: 675–680.
Kudora, K. (1991). Mechanism of cavitation development in the pine wilt disease. European Journal of Forest Pathology 21: 82–89.
Kuroda, K., Yamada, T. and Ito, S. (1991). Bursaphelenchus xylophilus induced pine wilt: factors associated with resistance. European Journal of Forest Pathology 21: 430–438.
Mamiya, Y. (1985). Initial pathological changes and disease development in pine trees inoculated by the pine wood nematode, Bursaphelenchus xylophilus. Annals Pathology of the Society of Japan 51: 546–555.
Mamiya, Y. (1999). Review on the pathogenicity of Bursaphelenchus mucronatus. In Sustainability of Pine Forests in Relation to Pine Wilt and Decline. Proceedings of an International Symposium, Tokyo, Japan, 26–30 October 1998 (eds. K. Futai, K. Togashi and T. Ikeda), pp. 57–64. Shokado Shote, Kyoto, Japan.
Polomski, J., Schönfeld, U., Braasch, H., Dobbertin, M., Burgermeister, W. and Rigling, D. (2006). Occurrence of Bursaphelenchus species in declining Pinus sylvestris in a dry Alpine valley in Switzerland. Forest Pathology 36: 110–118.
Riga, E., Sutherland, J. and Webster, J. (1991). Pathogenicity of pine wood nematode isolates and hybrids to Scots pine seedlings. Nematologica 37: 285–292.
Rutherford, T., Riga, E. and Webster, J. (1992). Temperature-mediated behavioral relationships in Bursaphelenchus xylophilus, B. mucronatus and their hybrids. Journal of Nematology 24: 40–44.
Skarmoutsos, G. and Michalopoulos-Skarmoutsos, H. (2000). Pathogenicity of Bursaphelenchus sexdentati, Bursaphelenchus leoni and Bursaphelenchus hellenicus on European pine seedlings. Forest Pathology 30: 149–156.
Suzuki, K. (2004). Pine wilt disease – a threat to pine forests in Europe. In The pine wood nematode, Bursaphelenchus xylophilus. Nematology Monographs and Perspectives, vol. 1 (eds. M. Mota and P. Vieira), pp. 25–30. E. J. Brill, Leiden, The Netherlands.
Utsuzawa, S., Fukuda, K. and Sakaue, D. (2005). Use of magnetic resonance microscopy for the non-destructive observation of xylem cavitation caused by pine wilt disease. Phytopathology 95: 737–743.
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Polomski, J., Rigling, D., Schweingruber, F. (2008). Development of External and Internal Symptoms in Pine Seedlings (Pinus sylvestris) Due to Inoculation with Bursaphelenchus vallesianus. In: Mota, M.M., Vieira, P. (eds) Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8455-3_28
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DOI: https://doi.org/10.1007/978-1-4020-8455-3_28
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