Abstract
Bursaphelenchus xylophilus is causal agent of the pine wilt disease, one of the major conifer diseases worldwide. This nematode is a quarantine organism in the European Union, where it has recently been discovered in a restricted area in Portugal. In our laboratory, research has been developed to characterize the genetic diversity of the nematode, and to provide a more comprehensive view of the relationships between B. xylophilus and the non-pathogenic Bursaphelenchus species. For that purpose, repetitive sequences known as satellite DNA (satDNA) have been cloned and characterized from the genome of B. xylophilus. Its species-specific distribution and high copy number in the genome make this sequence a very promising tool for molecular identification of the nematode, as will be illustrated with results obtained in the laboratory. In particular, the recent development of a very sensitive satDNA based real-time PCR assay will be presented. Moreover, sequencing of monomers of the satDNA was performed and phylogenetic analyses were conducted to analyse the diversity and relationships (1) between B. xylophilus isolates sampled worldwide; (2) among B. xylophilus populations all collected in the infested area from Portugal. Results will be discussed in the context of the origin and evolution of the pine wood nematode complex in Europe.
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References
Bachmann, L., Schibel, J.M., Raab, M. and Sperlich, D. (1993). Satellite DNA as a taxonomic marker. Biochemical Systematics and Ecology 21: 3–11.
Beridze, T. (1986). Satellite DNA. Springer-Verlag, Berlin, 149 pp.
Burgermeister, W., Metge, K., Braasch, H. and Buchbach, E. (2005). ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology 13: 29–42.
Cao, A.X., Liu, X.Z., Zhu, S.F. and Lu, B.S. (2005). Detection of the pinewood nematode, Bursaphelenchus xylophilus, using a real-time polymerase chain reaction assay. Phytopathology 95: 566–571.
Castagnone, C., Abad, P. and Castagnone-Sereno, P. (2005). Satellite DNA-based species-specific identification of single individuals of the pinewood nematode Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). European Journal of Plant Pathology 112: 191–193.
Csink, A.K. and Henikoff, S. (1998). Something from nothing: The evolution and utility of satellite repeats. Trends in Genetics 14: 200–204.
Dover, G.A. (2002). Molecular drive. Trends in Genetics 18: 587–589.
François, C., Castagnone, C., Boonham, N., Tomlinson, J., Lawson, R., Hockland, S., Quill, J., Vieira, P., Mota, M. and Castagnone-Sereno, P. (2007). Satellite DNA as a target for TaqMan real.time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus. Molecular Plant Pathology 8: 803–809.
Grenier, E., Castagnone-Sereno, P. and Abad, P. (1997). Satellite DNA sequences as taxonomic markers in nematodes of agronomic interest. Parasitology Today 13: 398–401.
Heid, C.A., Stevens, J., Livack, K.J. and Williams, P.M. (1996). Real time quantitative PCR. Genome Research 6: 986–994.
Holland, P., Abramson, R.D., Watson, R. and Gelfand, D.H. (1991). Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences USA 88: 7276–7280.
Leroy, S., Duperray, C. and Morand, S. (2003). Flow cytometry for parasite nematode genome size measurement. Molecular and Biochemical Parasitology 128: 91–93.
Mamiya, Y. (2004) Pine wilt disease in Japan. In The pinewood nematode, Bursaphelenchus xylophilus. Nematology monographs and perspectives, vol. 1 (eds. M. Mota and P. Vieira). Leiden, The Netherlands, E.J. Brill, pp. 9–20.
Matsunaga, K. and Togashi, K. (2004). A simple method for discriminating Bursaphelenchus xylophilus and B. mucronatus by species-specific polymerase chain reaction primers. Nematology 6: 273–277.
Mestrovic, N., Castagnone-Sereno, P. and Plohl, M. (2006). Interplay of selective pressure and stochastic events directs evolution of the MEL172 satellite DNA library in root-knot nematodes. Molecular Biology and Evolution 23: 2316–2325.
Mota, M.M., Braasch, H., Bravo, M.A., Penas, A.C., Burgermeister, W., Metge, K. and Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727–734.
Ryss, A., Vieira, P., Mota, M. and Kulinich, O. (2005). A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology 7: 393–458.
Tarès, S., Lemontey, J.M., de Guiran, G. and Abad, P. (1993). Cloning and characterization of a highly conserved satellite DNA sequence specific for the phytoparasitic nematode Bursaphelenchus xylophilus. Gene 129: 269–273.
Tarès, S., Lemontey, J.M., de Guiran, G. and Abad, P. (1994). Use of a species-specific satellite DNA from Bursaphelenchus xylophilus as a diagnostic probe. Phytopathology 84: 294–298.
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Castagnone-Sereno, P., Castagnone, C., François, C., Abad, P. (2008). Satellite DNA as a Versatile Genetic Marker for Bursaphelenchus xylophilus. In: Mota, M.M., Vieira, P. (eds) Pine Wilt Disease: A Worldwide Threat to Forest Ecosystems. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8455-3_16
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DOI: https://doi.org/10.1007/978-1-4020-8455-3_16
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