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Variation in ITS and 28S rDNA of Bursaphelenchus Species (Nematoda: Parasitaphelenchidae)

  • Kai Metge
  • Helen Braasch
  • Jianfeng Gu
  • Wolfgang Burgermeister

Abstract

Internal transcribed spacers and 28S D2/D3 domain of rDNA were used to infer the phylogenetic relationships among Bursaphelenchus species, with special emphasis on members of the xylophilus and fungivorus groups. Sequence alignments and phylogenetic analysis using neighbour-joining and maximum parsimony algorithms resulted in trees with similar topologies. The 17 Bursaphelenchus species examined can be separated into two main branches: the first includes members of the xylophilus group and the second includes the species of the fungivorus group, separated from the remaining species B. eremus, B. hofmanni, B. rainulfi and B. yongensis. As far as known, the species of the xylophilus group are phoretically associated with longhorn beetles. The phylogenetic analysis revealed the digger bee associated B. abruptus as the basal taxon of the species investigated or at least of the xylophilus group. The remainder of the species are, as far as known, associated with bark beetles and a soil-dwelling bee in case of B. seani. The significantly supported groups are largely

Keywords

Maximum Parsimonious Lateral Line Bark Beetle Pine Wilt Disease Longhorn Beetle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anthoine, G. and Mugniery, D. (2005). Variability of the ITS rDNA and identification of Nacobbus aberrans (Thorne, 1935) Thorne and Allen, 1944 (Nematoda: Pratylenchidae) by rDNA amplification. Nematology 7: 503–516.CrossRefGoogle Scholar
  2. Beckenbach, K., Blaxter, M. and Webster, J.M. (1999). Phylogenetic of Bursaphelenchus species derived from analysis of ribosomal internal transcribed spacer DNA sequences. Nematology 1: 539–548.CrossRefGoogle Scholar
  3. Blok, V.C., Malloch, G., Harrower, B., Phillips, M.S. and Vrain T.C. (1998). Intraspecific variation in ribosomal DNA in populations of the potato cyst nematode Globodera pallida. Journal of Nematology 30: 262–273.Google Scholar
  4. Boutsika, K., Brown, D.J.F., Phillips, M.S. and Blok, V.C. (2004). Molecular characterisation of the ribosomal DNA of Paratrichodorus macrostylus, P. pachydermus, Trichodorus primitivus and T. similis (Nematoda: Trichodoridae). Nematology 6:641–654.CrossRefGoogle Scholar
  5. Braasch, H. (2001). Bursaphelenchus species in conifers in Europe: distribution and morphological relationships. European and Mediteranean Plant Protection Organisation, OEPP/EPPO, Bulletin 31: 127–142.Google Scholar
  6. Braasch, H., Burgermeister, W., Schröder, T., Apel, K.-H. and Brandstetter, M. (2006). Occurrence of Bursaphelenchus eremus Rühm, 1956 (Nematoda: Parasitaphelenchidae) on oaks in State Brandenburg/Germany and supplementary species description. Nachrichtenblatt für den Deutschen Pflanzenschutzdienst 58: 148–153.Google Scholar
  7. Braasch, H., Metge, K. and Burgermeister, W. (1999). Bursaphelenchus-Arten (Nematoda, Parasitaphelenchidae) in Nadelgehölzen in Deutschland und ihre ITS-RFLP-Muster. Nachrichtenblatt Deutscher Pflanzenschutzdienst 51: 312–320.Google Scholar
  8. Burgermeister, W., Metge, K., Braasch, H. and Buchbach, E. (2005). ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology 13: 29–42.Google Scholar
  9. Giblin-Davis, R.M., Hazir, S., Center, B.J., Ye, W., Keskin, N., Thorp, R.W. and Thomas, K. (2005). Bursaphelenchus anatolius n. sp. (Nematoda: Parasitaphelenchidae), an associate of bees in the genus Halictus. Journal of Nematology 37: 336–342.Google Scholar
  10. Giblin-Davis, R.M., Mundo-Ocampo, M., Baldwin, J.G., Norden, B.B. and Batra, S.W.T. (1993). Description of Bursaphelenchus abruptus n. sp. (Nemata: Aphelenchoididae), an associate of a digger bee. Journal of Nematology 25: 161–172.Google Scholar
  11. Giblin, R.M. and Kaya, H.K. (1983). Bursaphelenchus seani n. sp. (Nematoda, Aphelenchoididae), a phoretic associate of Anthophora bomboides stanfordiana Cockerell, 1904 (Hymenoptera, Anthophoridae). Revue de Nématologie 6: 39–50.Google Scholar
  12. Gu, J., Braasch, H., Burgermeister, W., Brandstetter, M. and Zhang, J. (2006). Description of Bursaphelenchus yongensis sp.n. (Nematoda: Parasitaphelenchidae) isolated from Pinus massoniana in China. Russian Journal of Nematology 14: 91–99.Google Scholar
  13. Hall, T.A. (1999). BioEdit: a user-friendly biological sequences alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium 41: 95–98.Google Scholar
  14. Iwahori, H., Tsuda, K., Kanzaki, N., Izui, K. and Futai, K. (1998). PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease. Fundamental and Applied Nematology 21: 655–666.Google Scholar
  15. Kang, J.S., Choi, K.S., Shin, S.C., Moon, I.S., Lee, S.G. and Lee, S.H. (2004). Development of an efficient PCR-based diagnosis protocol for the identification of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). Nematology6: 279–285.CrossRefGoogle Scholar
  16. Kanzaki, N. and Futai, K. (2002). A PCR primer set for determination of phylogenetic relationships of Bursaphelenchus species within the xylophilus group. Nematology 4: 35–41.CrossRefGoogle Scholar
  17. Kumar, S., Tamura, K. and Nei, M. (2004). MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5: 150–163.PubMedCrossRefGoogle Scholar
  18. Matsunaga, K. and Togashi, K. (2004). A simple method for discriminating Bursaphelenchus xylophilus and B. mucronatus by species-spezific polymerase chain reaction primer pairs. Nematology 6: 273–277.CrossRefGoogle Scholar
  19. Metge, K., Braasch, H., Gu, J. and Burgermeister, W. (2006). Interspecific variation in ITS rDNA of Bursaphelenchus species (Nematoda: Parasitaphelencidae) of different groups. Russian Journal of Nematology 14: 147–158.Google Scholar
  20. Metge, K. and Burgermeister, W. (2005). Multiple displacement amplification of DNA for ITS-RFLP analysis of Bursaphelenchus individual nematodes. Nematology7: 253–257.CrossRefGoogle Scholar
  21. Mota, M.M., Braasch, H., Bravo, M.A., Penas, A.C., Burgermeister, W., Metge, K. and Sousa, E. (1999). First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727–734.CrossRefGoogle Scholar
  22. Powers, T.O., Todd, T.C., Burnell, A.M., Murray, P.C.B., Fleming, C.C., Szalanski, A.L., Adams, B.A. and Harris, T.S. (1997). The rDNA internal transcribed spacer region as a taxonomic marker for nematodes. Journal of Nematology 29: 441–450.Google Scholar
  23. Ryss, A., Vieira, P., Mota, M. and Kulinich, O. (2005). A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology 7: 393–458.CrossRefGoogle Scholar
  24. Schönfeld, U., Braasch, H. and Burgermeister, W. (2006). Bursaphelenchus spp. (Nematoda: Parasitaphelenchidae) in wood chips from sawmills in Brandenburg and description of Bursaphelenchus willibaldi sp. n. Russian Journal of Nematology 14:119–126.Google Scholar
  25. Subbotin, S.A., Halford, P.D., Warry, A. and Perry, R.N. (2000). Variation in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants. Nematology 2: 591–604.CrossRefGoogle Scholar
  26. Zheng, J., Subbotin, S.A., He, S., Gu, J. and Moens, M. (2003). Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology 11: 17–22.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kai Metge
    • 1
  • Helen Braasch
  • Jianfeng Gu
  • Wolfgang Burgermeister
  1. 1.Institute for Biosafety of Genetically Modified Plants Julius Kuehn-Institute (JKI) Federal Research Centre for Cultivated PlantsQuedlinburgGermany

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