Debaryomyces hansenii: An Osmotolerant and Halotolerant Yeast

  • Monika Aggarwal
  • Alok K. Mondal

The yeast Debaryomyces hansenii which was isolated from saline environments such as sea water, concentrated brines, salty food, is one of the most halotolerant species. It can grow in media containing as high as 4 M NaCl, while the growth of Saccharomyces cerevisiae is limited in media with more than 1.7 M NaCl. This species is very important for food industry as it is used for surface ripening of cheese and meat products. In the recent past, there is growing interest in understanding the molecular mechanisms of high halotolerance exhibited by D. hansenii. Availability of genome sequence of D. hansenii has opened up new vistas in this direction


Debaryomyces hansenii halotolerance saline environment halophily osmotolerance 


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  1. Aelst, L., van Hohmann, B., Bulaya, B., Koning, W., de Sierksira, L., Neves, M.J., Luyten, K., and Alijo, R. 1993. Mol. Microbiol. 8: 927–943.CrossRefGoogle Scholar
  2. Adler, L., Blomberg, A. and Nilsson, A. 1985. J. Bacteriol. 162: 300–306.Google Scholar
  3. Adler, L. and Gustafsson, L. 1980. Arch. Microbiol. 124: 123–130.CrossRefGoogle Scholar
  4. Aggarwal, M., Bansal, P.K. and Mondal, A.K. 2006. Yeast 22: 457–470.CrossRefGoogle Scholar
  5. Aggarwal, M. and Mondal, A.K. 2006. Eukaryotic Cell 5: 262–271.CrossRefGoogle Scholar
  6. Albertyn, J., Hohmann, S., Thevelein, J.M. and Prior, B.A. 1994. Mol. Cell Biol. 14: 4135–4144.Google Scholar
  7. Almagro, A., Prista C., Benito B., Loureiro-Dias M.C., Ramos J. 2001. J. Bact. 183: 3251–3255.CrossRefGoogle Scholar
  8. Almagro, A., Prista, C., Castro, S., Quintas, C., Madeira-Lopes, A., Ramos, J. and Loureiro-Dias, M.C. 2000. Int. J. Food Microbiol. 56: 191–197.CrossRefGoogle Scholar
  9. Arfi, K., Spinnle, H.E., Tache, R. and Bonnarme, P. 2002. Appl. Microbiol. Biotechnol. 58: 503–510.CrossRefGoogle Scholar
  10. Banno, I. and Mikata, K. 1985. Inst. Ferment. Osaka Res. Commun. 12: 63–69.Google Scholar
  11. Bansal, P.K. and Mondal, A.K. 2000. Yeast 16: 81–88.CrossRefGoogle Scholar
  12. Bansal, P.K., Sharma, P. and Mondal, A.K. 2001. Yeast 18: 1207–1216.CrossRefGoogle Scholar
  13. Banuelos, M.A., Ramos, J., Calero, F., Braun, V. and Potier, S. 2002. Yeast 19: 1365–1372.CrossRefGoogle Scholar
  14. Banuleos, M.A., Sychrova, H., Blekyasten-Grosshans, C., Souciet, J.L. and Potier, S. 1998. Microbiology 144: 2749–2758.Google Scholar
  15. Banuett, F. 1998. Microbiol. Mol. Biol. Rev. 62: 249–274.Google Scholar
  16. Barnett, J.A., Payne, R.W. and Yarrow, D. 2000. In: Yeasts: Characteristics and Identification, 3rd edn. (eds. Barnett J.A., Payne, R.W. and Yarrow, D), Cambridge University Press, Cambridge.Google Scholar
  17. Besancon, X., Smet C., and Chabalier, C. 1992. Int. J. Food Microbiol. 17: 9–18.CrossRefGoogle Scholar
  18. Blomberg, A. and Adler, L. 1989. J. Bacteriol. 171: 1087–1092.Google Scholar
  19. Bolumar, T., Sanz, Y., Aristoy, M.-C. and Toldra, F. 2003a. Appl. Environ. Microbiol. 69: 227–232.CrossRefGoogle Scholar
  20. Bolumar, T., Sanz, Y., Aristoy, M.-C. and Toldra, F. 2003b. Int. J. Food. Microbiol. 86: 141–151.CrossRefGoogle Scholar
  21. Borst-Pauwels, G.W. 1981. Biochem. Biophys. Acta 650: 88–127.Google Scholar
  22. Breuer, U. and Harms, H. 2006. Yeast 23: 415–437.CrossRefGoogle Scholar
  23. Brewester, J.L., de Valoir, T., Dweyer, N.D., Winter, E. and Gustin, M.C. 1993. Science 259: 1760–1763.CrossRefGoogle Scholar
  24. Brown, A.D. 1978. Adv. Microbiol. Physiol. 17: 181–242.CrossRefGoogle Scholar
  25. Butinar, L., Santos, S., Spencer-Martins, I. and Oren, A., Gunde- Cimerman, N. 2005. FEMS Microbiol. Lett. 244: 229–234.CrossRefGoogle Scholar
  26. Cai, J., Roberts, I.N. and Collins, M.D. 1996. Int. J. Syst. Bacteriol. 46: 542–549.Google Scholar
  27. Camacho, M., Ramos, J. and Rodriguez-Navaro, A. 1981. Curr. Microbial. 6: 295–299.CrossRefGoogle Scholar
  28. Converti, A. and Dominguez, J.M. 2001. Biotechnol. Bioeng. 75: 39–45.CrossRefGoogle Scholar
  29. Corredor, M., Davila, A.-M., Casaregola, S. and Gaillardin, C. 2003. Antonie van Leeuwenhoek 83: 215–222.CrossRefGoogle Scholar
  30. Corredor, M., Davila, A.M., Gaillardin, C. and Casaregola, S. 2000. FEMS Microbiol Lett. 193: 171–177.CrossRefGoogle Scholar
  31. Cruz, J.M., Domínguez, J.M., Domínguez, H. and Parajó, J.C. 2000. Biotech. Lett. 22: 605–610.CrossRefGoogle Scholar
  32. Davenport, R.R. 1980. In: Biology and Activities of Yeasts, (eds. Skinner, F.A., Passmore, S.M., and Davenport, R.R.) Academic Press, London, pp. 215–230.Google Scholar
  33. de Silóniz, M., Isabel, V., María-José, P. and José, M. 2000. J. Food Protection 63: 651–654.Google Scholar
  34. Deiana, P., Fatichenti F. and Farries G.A. 1984. Le Lait 64: 380–394.CrossRefGoogle Scholar
  35. Dujon, B., Sherman D., Fischer G., Durrens P., Casaregola S. and Lafontaine I. 2004. Nature 430: 35–44.CrossRefGoogle Scholar
  36. Dura, M.A., Flores, M. and Toldra, F. 2004a. Food Chem. 86: 385–389.CrossRefGoogle Scholar
  37. Dura, M.A., Flores, M. and Toldra, F. 2004b. Food Chem. 86: 391–399.CrossRefGoogle Scholar
  38. Dura, M.A., Flores, M. and Toldra, F. 2004c. Meat Sci. 68: 319–328.CrossRefGoogle Scholar
  39. Edgley, M. and Brown, A.D. 1983. J. Gen. Microbiol. 129: 3453–3464.Google Scholar
  40. Eriksson, P., Andre, L., Ansell, R., Blomberg, A. and Adler, L. 1995. Mol. Microbiol. 17: 95–107.CrossRefGoogle Scholar
  41. Fabre, E., Muller, H., Therizols, P., Lafontaine, I., Dujon, B. and Fairhead, C. 2004. Mol. Biol. Evolution. 22: 856–873.Google Scholar
  42. Fatichenti, F., Bergere, J.L., Deiana, P. and Farris, G.A. 1983. J. Dairy Res. 50: 449–457.Google Scholar
  43. Ferrando, A., Kron, S.J., Rios, G., Fink, G.R. and Serrano, R 1995. Mol. Cell. Biol. 15: 5470–5481.Google Scholar
  44. Ferrigno, P., Posas, F., Koepp, D., Saito, H. and Silver P.A. 1998. EMBO J. 17: 5606–5614.CrossRefGoogle Scholar
  45. Fleet, G.H. 1990. J. Appl. Bacteriol. 68: 199–211.Google Scholar
  46. Fleet, G.H. and Mian, M.A. 1987. Int. J. Food Microbiol. 4: 145–155.CrossRefGoogle Scholar
  47. Forrest, S.I., Robinow C.F. and Lachance M.A. 1987. Can. J. Microbiol. 33: 967–970.Google Scholar
  48. Gaber, R.F. 1992. Int. Rev. Cytol. 137: 299–353.CrossRefGoogle Scholar
  49. Gancedo, C. and Serrano, R. 1989. In: The Yeast, vol. 3, 2nd edn., (eds. Harrison, J.S. and Rose, A.H.), Academic Press, San Diego, CA., pp. 205–259.Google Scholar
  50. Garciadeblas, B., Rubio, F., Quintero, F.J., Banuleos, M.A. and Rodriguez-Navarro, A. 1993. Mol. Gen. Gene 236: 363–368.CrossRefGoogle Scholar
  51. Gaxiola, R., de Larrinoa, I.F., Villalba, J.M. and Serrano, R. 1992. EMBO J. 11: 3157–3164.Google Scholar
  52. Girio, F.M., Amaro, C., Azinheira, H., Pelica, F. and Amaral-Collaco, M.T. 2000. Biores. Technol. 71: 245–251.CrossRefGoogle Scholar
  53. Girio, F.M., Pelica, F. and Amaral-Collaco, M.T. 1996. Appl. Biochem. Biotechnol. 56: 79–87.CrossRefGoogle Scholar
  54. Girio, F.M., Roseiro, J.C. and Sa-Machado, P. 1994. Enzyme Microb. Technol. 16: 1074–1078.CrossRefGoogle Scholar
  55. Glaser, H.V., Thomas, D., Gaxiola, H., Montrichard, F., Surdin-Kerjan, Y. and Serrano, R. 1993. EMBO J. 12: 3105–3110.Google Scholar
  56. Gonzalez-Hernandez, J.C., Cardenas-Monroy, C.A. and Pena, A. 2004. Yeast 21: 403–412.CrossRefGoogle Scholar
  57. Gustin, M.C., Albertyn, J., Alexander, M. and Davenport, K. 1998. Microbiol. Mol. Biol. Rev. 62: 1264–1300.Google Scholar
  58. Haber, J.E. 1998. Annu. Rev. Genet. 32: 561–599.CrossRefGoogle Scholar
  59. Herskowitz, I., Rine, J. and Strathern, J. N. 1992. In: The Molecular Biology of the Yeast Saccharomyces: Gene Expression (eds. Broach, J.R., Pringle, J.R. and Jones, E.W.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N Y, pp. 583–656.Google Scholar
  60. Hertogh, B.D., Hancy, F., Goffeau, A. and Baret P.V. 2006. Genetics 172: 771–781.CrossRefGoogle Scholar
  61. Hirata, D., Harada, S., Namba, H. and Miyakawa, T. 1995. Mol. Gen. Genet. 249: 257–264.CrossRefGoogle Scholar
  62. Jia, Z. P., McCullough, N., Hemmingsen, S. and Young, P.G. 1992. EMBO J. 11: 1631–1640.Google Scholar
  63. Kinclova, O., Potier, S. and Sychrova, H. 2001a. FEBS Lett. 504: 11–15.CrossRefGoogle Scholar
  64. Kinclova, O., Potier, S. and Sychrova, H. 2001b. J. Biotechnol. 88: 151–158.CrossRefGoogle Scholar
  65. Kinclova, O., Potier, S. and Sychrova, H. 2002. Microbiology. 148: 1225–1232.Google Scholar
  66. Kreger van Rij, N.J. and Veenhuis, M. 1975. J. Gen. Microbiol. 89: 256–264.Google Scholar
  67. Kurtzman, C.P. and Robnett, C.J. 1998. Antonie Van Leeuwenhoek 73: 331–371.CrossRefGoogle Scholar
  68. Kurtzman, C.P., Smiley, M.J. and Baker, F.L. 1975. Mycopathol. Mycol. Appl. 55: 29–34.Google Scholar
  69. Larsson, K., Ansell, R., Eriksson, P. and Adler, L. 1993. Mol. Microbiol. 10: 1101–1111.CrossRefGoogle Scholar
  70. Lepingle, A., Casaregola, S., Neuveglise, C., Bon, E., Nguyeh, H.V., and Artiguenave, F. 2000. FEBS Lett. 487: 82–86.CrossRefGoogle Scholar
  71. Lopez-Coronado, J.M., Belles, J.M., Lesage, F., Serrano, R. and Rodriguez, P.L. 1999. J. Biol. Chem. 274: 16034–16039.CrossRefGoogle Scholar
  72. Lucas, C., da Costa, M. and van Uden, N. 1990. Yeast 6: 187–191.CrossRefGoogle Scholar
  73. Madhani, H.D. and Fink, G.R. 1998. Trends Genet. 14: 151–155.CrossRefGoogle Scholar
  74. Maeda, T., Wurgler-Murphy, S.M. and Saito, H. 1994. Nature 369: 242–245.CrossRefGoogle Scholar
  75. Maeda, T., Takekawa M. and Saito H. 1995. Science 269: 554–558.CrossRefGoogle Scholar
  76. Mager, W.H. and Varela J.C. 1993. Mol. Microbiol. 10: 253–258.CrossRefGoogle Scholar
  77. Marquez, J.A. and Serrano R. 1996. FEBS Lett. 382: 89–92.CrossRefGoogle Scholar
  78. Martinez-Pastor M.T., Marchler G., Schuller C., Marchler-Bauer A., Ruis H. Estruch F. 1996. EMBO J. 15: 2227–2235.Google Scholar
  79. Martorell, P., Fernandez-Espinar, M.T. and Querol, A. 2005. FEMS Yeast Res. 12: 1157–1165.CrossRefGoogle Scholar
  80. Mattsson, R., Haemig, P.D. and Olsen, B. 1999. Med. Mycol. 37: 367–369.CrossRefGoogle Scholar
  81. Mortensen, H.D., Gori, K., Jespersen, L. and Arneborg, N. 2005. FEMS Microbiol. Lett. 249: 165–170.CrossRefGoogle Scholar
  82. Mounier, J., Gelsomino R., Georges S., Vancanneyt, M. and Vandemeubroecke, K. 2005. Microbiol. 71: 6489–6500.Google Scholar
  83. Mulet, J.M., Leube, S.J., Kron, G., Fink, G.R. and Serrano, R. 1999. Mol. Cell Biol. 19: 3328–3337.Google Scholar
  84. Murguia, J.R., Belles, J.M. and Serrano, R. 1995. Science 267: 232–234.CrossRefGoogle Scholar
  85. Murguia, J.R., Belles, J.M. and Serrano, R. 1996. J. Biol. Chem. 271: 29029–29033.CrossRefGoogle Scholar
  86. Nakase, T. and Suzuki, M. 1985. J. Gen. Appl. Microbiol. 31: 71–86.CrossRefGoogle Scholar
  87. Nakase, T., Suzuki, M., Phaff, H.J. and Kurtzman, C.P. 1998. In: The Yeasts — A Taxonomic Study (eds. Kurtzman, C.P. and Fell, J.W.), Appl. Microbiol. 31: 71–86.CrossRefGoogle Scholar
  88. Neves, M.L., Oliveria, R.P. Lucas, C.M. 1997. Microbiology 143: 1133–1139.Google Scholar
  89. Nilsson, A. and Adler, L. 1990. Biochem. Biophys. Acta 1034: 180–185.Google Scholar
  90. Nobre, F.M. and DaCosta, M.S. 1985. Can. J. Micobiol. 31: 1061–1064.CrossRefGoogle Scholar
  91. Norbeck, J., Pahlman, A.K., Akhtar N., Blomberg, A. and Adler, L. 1996. J. Biol. Chem. 271: 13875–13881.CrossRefGoogle Scholar
  92. Norkans, B. 1968. Arch. Mikrobiol. 62: 358–372.CrossRefGoogle Scholar
  93. Norkrans, B. 1966. Arch. Mikrobiol. 54: 374.CrossRefGoogle Scholar
  94. Norkrans, B. and Kylin, A. 1969. J. Bacteriol. 100: 836–845.Google Scholar
  95. Oliviera, R., Lages, F., Silva-Graca, C. and Lucas, C. 2003. Biochim. Biophys. Acta 1613: 57–71.CrossRefGoogle Scholar
  96. Olz, R., Larsson, K., Adler, L. and Gustafsson, L. 1993. J. Bacteriol. 175: 2205–2213.Google Scholar
  97. Onishi, H. 1963. Adv. Food Res. 12: 53–94.Google Scholar
  98. Parajo, J.C., Dominguez, H. and Dominguez. J.M. 1997. Enz. Microb. Technol. 21:18–24.CrossRefGoogle Scholar
  99. Petersen, K.M. and Jespersen, L. 2004. J. Appl. Microbiol. 97: 205–213.CrossRefGoogle Scholar
  100. Petersen, K.M., Moller, P.L. and Jespersen, L. 2001. Int. J. Food Microbiol. 69: 11–24.CrossRefGoogle Scholar
  101. Phaff, H.J., Martini, A.V. and Starmer, W.T. 1998. Int. J. Syst. Bacteriol. 48: 1419–1424.Google Scholar
  102. Posas, F. and Saito H. 1997. Science 276: 1702–1705.CrossRefGoogle Scholar
  103. Posas, F., Wurgler-Murphy S.M., Maeda T., Witten E.A., Thai T.C. and Saito H. 1996. Cell 86: 865–875.CrossRefGoogle Scholar
  104. Prillinger, H., Molnar, O., Eliskases-Lechner, F. and Lopandic, K. 1999. Antonie van Leeuwenhoek 75: 267–83.CrossRefGoogle Scholar
  105. Prista, C., Alamagro, A., Loureiro-Dias, M. and Ramos, J. 1997. Appl. Environ. Microbiol. 63: 4005–4009.Google Scholar
  106. Prista, C., Alamagro, A., Loureiro-Dias, M. and Ramos, J. 1998. Folia Microbiol. (Praha) 43: 212–214.CrossRefGoogle Scholar
  107. Prista, C., Loureiro-Dias M.C., Montiel V., Garcia R. and Ramos J. 2005. FEMS Yeast Res. 5: 693–701.CrossRefGoogle Scholar
  108. Prista, C., Soeiro A., Vesely P., Almagro A., Ramos J. and Loureiro-Dias M.C. 2002. FEMS Yeast Res. 2: 151–157.Google Scholar
  109. Quintero, F.J., Garciadeblas B. and Rodriguez-Navarro A. 1996. Plant Cell 8: 529–537.CrossRefGoogle Scholar
  110. Raitt, D.C., Posas F. and Saito H. 2000. EMBO J. 19: 4623–4631.CrossRefGoogle Scholar
  111. Reiser, V., Ruis H. Ammerer G. 1999. Mol. Biol. Cell 10: 1147–1161.Google Scholar
  112. Romano, A., Casaregola, S., Torre, P. and Gaillardin, C. 1996. System Appl. Microbiol. 19: 255–264.Google Scholar
  113. Roostita, R. and Fleet G.H. 1996. Int. J. Food Microbiol. 28: 393–404.CrossRefGoogle Scholar
  114. Saldanha-da-gama, A., Malfeito-Ferrira, M. and Lureiro, V. 1997. Int. J. Food. Microbiol. 37: 201–207.CrossRefGoogle Scholar
  115. Sanchez, N.S., Calahorra, M. Gonzalez-Hernandez, J.C. and Pena, A. 2006. Yeast 23: 361–374.CrossRefGoogle Scholar
  116. Seiler, H. and Busse, M. 1990. Int. J. Food. Microbiol. 11: 289–303.CrossRefGoogle Scholar
  117. Sharma, P., Meena, N., Aggarwal, M. and Mondal, A.K. 2005. Curr. Genet. 48: 162–170.CrossRefGoogle Scholar
  118. Sharma, P. and Mondal, A.K. 2005. Biochem. Biophysi. Res. Commun. 328: 906–913.CrossRefGoogle Scholar
  119. Sharma, P. and Mondal, A.K. 2006. Biochem. Biophys. Res. Commun. 346: 562–566.CrossRefGoogle Scholar
  120. Sugita, T. and Nakase, T. 1999. Syst. Appl. Microbiol. 22: 79–86.Google Scholar
  121. Sychrova, H. 2004. Physiol. Res. 53: 91–98.Google Scholar
  122. Tekaia, F., Blandin, G., Malpertuy, A., Liorente, B., Durrens, P., and Toffano-Nioche, O. 2000. FEBS Lett. 487: 17–30.CrossRefGoogle Scholar
  123. Thome, P.E. 2004. Yeast 21: 119–126.CrossRefGoogle Scholar
  124. Thome, P.E. and Trench, R.K. 1999. Mar. Biotechnol. 1: 230–238.CrossRefGoogle Scholar
  125. Thome-Oritz, P.E., Pena, A. and Ramirez, J. 1998. Yeast 14: 1355–1371.CrossRefGoogle Scholar
  126. Tilbury, R.H. 1980. In: Biology and Activities of Yeasts (eds. Skinner, F.A., Passmore, S.M. and Davenport, R.R.), Academic Press, London, pp. 153–176.Google Scholar
  127. van den Tempel, T. and Jacobsen, M. 2000. Int. Dairy J. 10: 263–270.CrossRefGoogle Scholar
  128. van der Walt, J.P., and Taylor, M.B. and Liebenberg N.V. 1977. Antonie van Leeuwenhoek 43: 205–218.CrossRefGoogle Scholar
  129. van Uden, N. and Fell, J.W. 1968. Adv. Microbiol. Sea 1: 167–201.Google Scholar
  130. Velkova, K. and Sychrova, H. 2006. Gene 369: 27–34.CrossRefGoogle Scholar
  131. Viega, A., Arabaca, J.D. and Loureiro-Dias, M.C. 2003a. FEMS Yeast Res. 3: 239–245.CrossRefGoogle Scholar
  132. Viega, A., Arabaca, J.D. and Loureiro-Dias, M.C. 2003b. J. Appl. Microbiol. 95: 364–371.CrossRefGoogle Scholar
  133. Veiga, A., Arrabaca, J.D., Sansonetty, F., Ludovico, P., Corte-Real, M. and Loureiro-Dias, M.C. 2003c. FEMS Yeast Res. 3: 141–148.CrossRefGoogle Scholar
  134. Wagner, D., Sander, A., Bertz, H., Finke, J. and Kern, W.V. 2005. Infection 33: 397–400.CrossRefGoogle Scholar
  135. Wenning, M., Seiler, H. and Scherer, S. 2002. Appl. Environ. Microbiol. 68: 4717–4721.CrossRefGoogle Scholar
  136. Wieland, J., Nitsche, A.M., Strayle, J., Steiner, H. and Rudolph, H.K. 1995. EMBO J. 14: 3870–3882.Google Scholar
  137. Wilmotte, A., van de Peer, Y., Goris, A., Chapelle, S., de Baere, R., Nelissen, B., Neefs, J.-M., Hennebert, G.L. and de Wachter, R. 1993 Syst. Appl. Microbiol. 16: 436–444.Google Scholar
  138. Wong, B., Kiehn, T.E., Edwards, F., Bernard, E.M., Marcove, R.C., de Harven, E. and Armstrong, D. 1982. J. Clin. Microbiol. 16: 545–548.Google Scholar
  139. Xiong, L., Lee, B., Ishitani, M., Lee, H., Zhang, C. and Zhu, J.K. 2001. Genes Dev. 15: 1971–1984.CrossRefGoogle Scholar

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Authors and Affiliations

  • Monika Aggarwal
  • Alok K. Mondal

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