Characterization of Bradyrhizobium elkanii Type III Secretion System
Type III secretion systems (T3SS) have been identified in a number of rhizobia and were shown to affect symbiosis with legumes. The genes encoding the conserved core of the T3SS (rhc; rhizobium conserved) are highly conserved in all cases, although differences exist in the secreted proteins. In our preliminary study, the presence of rhcC2 and rhcJ in Bradyrhizobium elkanii was verified by PCR and sequencing of the fragments. In this study, we characterized the T3SS of B. elkanii USDA61 by DNA sequencing, mutagenesis, RT-PCR and extracellular protein analysis.