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Defining the Roles PII and AmtB Proteins in the NH4+-Induced Post-Translational Control of Nitrogenase Activity

  • L. F. Huergo
  • M. Merrick
  • L. S. Chubatsu
  • F. O. Pedrosa
  • M. B. Steffens
  • E. M. Souza
Conference paper
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 42)

In some diazothophs, such as Azospirillum brasilense and Rhodospirillum rubrum, nitrogenase is post-translationally controlled by ADP-ribosylation of nitrogenase reductase (NifH). In response to addition of NH4+ to the medium, an ADP-ribose is linked to NifH resulting in nitrogenase inactivation (switch-off). This process is catalyzed by the DraT enzyme. When the added NH4+ is exhausted by cellular metabolism, the ADPribose group is removed by the DraG enzyme leading to nitrogenase activation (switch-on). The activities of both DraT and DraG are regulated by the cellular nitrogen status, although the signaling pathway is not fully understood.

Keywords

Biological Nitrogen Fixation Azospirillum Brasilense Rhodospirillum Rubrum DraG Activity DraG Localization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media B.V. 2008

Authors and Affiliations

  • L. F. Huergo
    • 1
  • M. Merrick
    • 2
  • L. S. Chubatsu
    • 1
  • F. O. Pedrosa
    • 1
  • M. B. Steffens
    • 1
  • E. M. Souza
    • 1
  1. 1.Departamento de Bioquímica e Biologia MolecularUniversidade Federal do ParanáCuritibaBrazil
  2. 2.Department of Molecular MicrobiologyJohn Innes CentreNorwichUK

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