X-ray Scattering for Bio-Molecule Structure Characterization

  • David M. Tiede
  • Xiaobing Zuo
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 26)

One of the challenges in photosynthesis research lies in resolving the molecular basis for function with atomic-scale precision across the full time-scale of photosynthetic events. While crystallography provides the most detailed and accurate measurement of molecular structure, structure determination is necessarily confi ned to specifi c crystalline states. Critical issues that can be left unresolved by crystallography include the range, amplitudes, and time-scales of molecular motions, and a determination of how these dynamic events are linked to biological function. Advances in synchrotron X-ray scattering techniques offer new opportunities for characterization of photosynthetic structure and dynamics in non-crystalline media that build upon crystallographic, NMR, and molecular dynamics databases, but are applied to conditions most closely relevant to in-situ function. Recent advances in the application of synchrotron X-ray scattering techniques include the extension to the high-angle, high-resolution domain, where measurements can be routinely made to a spatial resolution of 1 Å or better, and the development of coordinate-based analyses of X-ray scattering data that allows scattering data to be analyzed in terms of detailed coordinate models for structure and dynamics. Promising new directions include application of element specifi c anomalous X-ray scattering and ultrafast time-resolved scattering techniques. This chapter will review recent progress in the application of synchrotron scattering techniques for the in-situ characterization of molecular structure. This work has focused primarily on proof-of-principle and technique development using model compounds and molecular systems. These studies demonstrate opportunities for using in-situ solution diffraction for the resolution of conformational landscapes for photosynthetic complexes in non-crystalline states and for extending these studies to the picosecond time domain using pulsed synchrotron techniques.


Scatter Pattern Scattering Pattern Bacterial Reaction Center Linac Coherent Light Source Photoactive Yellow Protein 
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Copyright information

© Springer Science+Business Media B.V 2008

Authors and Affiliations

  • David M. Tiede
    • 1
  • Xiaobing Zuo
    • 1
  1. 1.Chemistry DivisionArgonne National LaboratoryArgonneUSA

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